Matrix solid-phase dispersion coupled with homogeneous ionic liquid microextraction for the determination of sulfonamides in animal tissues using high-performance liquid chromatography.
Matrix solid-phase dispersion coupled with homogeneous ionic liquid microextraction for the determination of sulfonamides in animal tissues using high-performance liquid chromatography.
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DOI：
10.1002/jssc.201500777
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发表时间：
2015-12
影响因子：
3.1
通讯作者：
Zhibing Wang;M. He;Chunzhu Jiang;Fengqing Zhang;Shanshan Du;Wen Feng;Hanqi Zhang
中科院分区：
文献类型：
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作者：
Zhibing Wang;M. He;Chunzhu Jiang;Fengqing Zhang;Shanshan Du;Wen Feng;Hanqi Zhang
建立了基质固相分散-均相离子液体微萃取联用技术，并将其应用于动物组织中磺胺类药物磺胺类药物的萃取。采用高效液相色谱法对目标分析物进行了分离和测定。固体样品直接用基质固相分散处理，洗脱液用均相离子液体微萃取处理。该方法采用离子液体作为萃取剂，可提高目标分析物的回收率。为避免使用有机溶剂，减少环境污染，采用水作为基质固相分散的洗脱溶剂。考察了离子液体的种类和体积、分散剂的种类、样品与分散剂的比例、洗脱溶剂的pH值、洗脱溶剂的体积、洗脱液中的盐量、离子对试剂(NH4PF6)的用量、离心时间等实验参数对回收率的影响。用于动物组织的分析，加标回收率为85.4%~118.0%，相对标准偏差小于9.30%。分析物的检出限为4.3~13.4μg/kg。
Matrix solid-phase dispersion coupled with homogeneous ionic liquid microextraction was developed and applied to the extraction of some sulfonamides, including sulfamerazine, sulfamethazine, sulfathiazole, sulfachloropyridazine, sulfadoxine, sulfisoxazole, and sulfaphenazole, in animal tissues. High-performance liquid chromatography was applied to the separation and determination of the target analytes. The solid sample was directly treated by matrix solid-phase dispersion and the eluate obtained was treated by homogeneous ionic liquid microextraction. The ionic liquid was used as the extraction solvent in this method, which may result in the improvement of the recoveries of the target analytes. To avoid using organic solvent and reduce environmental pollution, water was used as the elution solvent of matrix solid-phase dispersion. The effects of the experimental parameters on recoveries, including the type and volume of ionic liquid, type of dispersant, ratio of sample to dispersant, pH value of elution solvent, volume of elution solvent, amount of salt in eluate, amount of ion-pairing agent (NH4 PF6 ), and centrifuging time, were evaluated. When the present method was applied to the analysis of animal tissues, the recoveries of the analytes ranged from 85.4 to 118.0%, and the relative standard deviations were lower than 9.30%. The detection limits for the analytes were 4.3-13.4 μg/kg.