Identification and confirmation of three neutral alleles conferring wide compatibility in inter-subspecific hybrids of rice (Oryza sativa L.) using near-isogenic lines
Identification and confirmation of three neutral alleles conferring wide compatibility in inter-subspecific hybrids of rice (Oryza sativa L.) using near-isogenic lines
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DOI：
10.1007/s00122-005-2055-z
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发表时间：
2005-06
影响因子：
5.4
通讯作者：
Gejiao Wang;Yuqing He;C. Xu;Qifa Zhang
中科院分区：
文献类型：
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作者：
Gejiao Wang;Yuqing He;C. Xu;Qifa Zhang
广亲和性品种是一类特殊的水稻种质资源，它与籼、粳亚种均能产生可育杂种。以前的研究确定'Dular'和02428是两个WCV，并确定了一些QTL对亚种间杂种育性有很大的影响。本研究以“Dular”和02428为供体，“珍汕97”为受体，通过回交和分子标记辅助选择，获得了5个含有f5、f6和S5 3个QTL的近等基因系。三个近等基因系各携带一个渐渗等位基因，两个近等基因系各携带两个渐渗等位基因的组合。用这些近等基因系分别与指示种‘南京11’和粳稻‘巴利拉’测交。结果表明，来自“Dular”的f5等位基因（f5-Du）是广亲和性的中性等位基因，对花粉育性和小穗育性的影响较大;来自“Dular”的f6等位基因（f6-Du）是小穗育性的中性等位基因，对小穗育性的影响较小。来自02428（S5-08）的S5等位基因被证实是小穗育性的中性等位基因。根据f6和S5的基因组定位和效应推测，它们可能是同一个基因座。结果还表明，在典型的籼粳交中，不同位点的两个中性等位基因即使在组合中也不能产生正常的育性杂种。本文还讨论了这些结果对水稻育种的影响。
Wide-compatibility varieties (WCVs) are a special class of rice germplasm that is able to produce fertile hybrids when crossed to bothindicaandjaponicasubspecies. Previous studies determined ‘Dular’ and 02428 as two WCVs and identified a number of QTLs as having large effects on fertility of inter-subspecific hybrids. In this study, we developed five near-isogenic lines (NILs) for three of the QTLs,f5,f6andS5, by backcrossing and marker-assisted selection, using ‘Dular’ and 02428 as the donors and ‘Zhenshan 97’ as the recipient. Three of the NILs each carried one introgressed allele, and two NILs each carried two introgressed alleles in combinations. The NILs were testcrossed to anindicatester ‘Nanjing 11’ and ajaponicatester ‘Balilla’. The results showed that thef5allele from ‘Dular’ (f5-Du) is a neutral allele conferring wide compatibility, with a large effect on both pollen and spikelet fertility, and thef6allele from ‘Dular’ (f6-Du) is a neutral allele for spikelet fertility with smaller effect. TheS5allele from 02428 (S5-08) was confirmed to be a neutral allele for spikelet fertility. It is likely thatf6andS5are the same locus as deduced by their genomic locations and effects. The results also showed that even in combination, two neutral alleles of different loci were not able to produce normal fertility hybrids in typicalindica–japonicacrosses. The implications of the findings in rice breeding programs are discussed.