Optimizing modulation frequency for structured illumination in a fiber-optic microendoscope to image nuclear morphometry in columnar epithelium.
Optimizing modulation frequency for structured illumination in a fiber-optic microendoscope to image nuclear morphometry in columnar epithelium.
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DOI：
10.1364/boe.6.000870
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发表时间：
2015-03
影响因子：
3.4
通讯作者：
P. Keahey;T. Tkaczyk;Kathleen M Schmeler;R. Richards-Kortum
中科院分区：
文献类型：
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作者：
P. Keahey;T. Tkaczyk;Kathleen M Schmeler;R. Richards-Kortum
纤维光学显微镜已显示出承诺的图像核形态的变化，伴随着鳞状上皮组织，如在口腔粘膜和宫颈癌前病变的发展。然而，光纤显微内窥镜图像对比度受到组织内散射产生的散焦光的限制。具有柱状上皮的组织的散射系数可能大于鳞状上皮的散射系数，导致图像质量降低。为了解决这一挑战，我们提出了一种小型便携式显微内窥镜系统，能够使用结构化照明（SI）实时进行光学切片。开发了几种光学模型，并用于量化系统的切片能力。然后离体成像宫颈组织标本的柱状上皮，我们证明，除了SI实现更高的图像对比度，使可视化的核形态。
Fiber-optic microendoscopes have shown promise to image the changes in nuclear morphometry that accompany the development of precancerous lesions in tissue with squamous epithelium such as in the oral mucosa and cervix. However, fiber-optic microendoscopy image contrast is limited by out-of-focus light generated by scattering within tissue. The scattering coefficient of tissues with columnar epithelium can be greater than that of squamous epithelium resulting in decreased image quality. To address this challenge, we present a small and portable microendoscope system capable of performing optical sectioning using structured illumination (SI) in real-time. Several optical phantoms were developed and used to quantify the sectioning capabilities of the system. Columnar epithelium from cervical tissue specimens was then imaged ex vivo, and we demonstrate that the addition of SI achieves higher image contrast, enabling visualization of nuclear morphology.