Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy
Simple and fast spectral domain algorithm for quantitative phase imaging of living cells with digital holographic microscopy
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DOI：
10.1364/ol.42.000227
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发表时间：
2017-01-15
期刊：
影响因子：
3.6
通讯作者：
Kemper, Bjoern
中科院分区：
文献类型：
--
作者：
Min, Junwei;Yao, Baoli;Kemper, Bjoern
提出了一种简单快速的数字全息显微镜（DHM）相差补偿方法，用于活细胞的定量相像成像。通过分析离轴全息图的频谱，相位像差可以自动补偿，而无需拟合或预先了解设置和/或对象。该方法计算简单有效，适用于高变量DHM系统的在线定量监测。活体NIH-3T3小鼠成纤维细胞的自动定量相位成像结果证明了该方法的有效性和可行性。(C) 2017年美国光学学会
We present a simple and fast phase aberration compensation method in digital holographic microscopy (DHM) for quantitative phase imaging of living cells. By analyzing the frequency spectrum of an off-axis hologram, phase aberrations can be compensated for automatically without fitting or pre-knowledge of the setup and/or the object. Simple and effective computation makes the method suitable for quantitative online monitoring with highly variable DHM systems. Results from automated quantitative phase imaging of living NIH-3T3 mouse fibroblasts demonstrate the effectiveness and the feasibility of the method. (C) 2017 Optical Society of America