SEQUENCE AND SECONDARY STRUCTURE OF THE COLICIN FRAGMENT OF BACILLUS STEAROTHERMOPHILUS 16S RIBOSOMAL-RNA
SEQUENCE AND SECONDARY STRUCTURE OF THE COLICIN FRAGMENT OF BACILLUS STEAROTHERMOPHILUS 16S RIBOSOMAL-RNA
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DOI：
10.1093/nar/9.19.4909
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
VANKNIPPENBERG, PH
中科院分区：
文献类型：
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作者：
VANCHARLDORP, R;VANKIMMENADE, AMA;VANKNIPPENBERG, PH
嗜热脂肪芽孢杆菌16S核糖体RNA 3'末端的转录后修饰残基的序列和位置已从细菌素处理切下的片段中确定。该片段含有 52 个核苷酸，而来自大肠杆菌的相应片段则含有 49 个核苷酸。大肠杆菌糖体。如之前发表的 (1)，额外的核苷酸存在于紧邻 3' 末端的 UCU 序列中。序列的其余部分与 E 的序列相同。由于主要发夹茎中的三个碱基对改变，大肠杆菌除了六个位置外。 B 的大肠菌素片段的 UV 熔化特性的测量。嗜热脂肪菌素与大肠杆菌相同片段的比较。大肠杆菌显示来自嗜热菌的 RNA 具有更稳定的二级结构。
The sequence and the position of post-transcriptionally modified residues of the 3′-terminal end ofBacillus stearothermophilus16S ribosomal RNA have been determined from the fragment that is cleaved off by bacteriocin treatment. The fragment contains 52 nucleotides, as compared to the 49 nucleotides of the corresponding fragment fromE. coliribosomes. The additional nucleotides are present in the sequence UCU very next to the 3′-terminus as was published earlier (1). The remainder of the sequence is identical to the one ofE. coliexcept at six positions, due to three basepair alterations in a major hairpin stem. Measurements of the UV melting properties of the colicin fragment fromB. stearothermophilusin comparison to the same fragment ofE. colishow that the RNA from the thermophile has a more stable secondary structure.