IDENTIFICATION OF A 2ND HUMAN RETINOIC ACID RECEPTOR
IDENTIFICATION OF A 2ND HUMAN RETINOIC ACID RECEPTOR
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DOI：
10.1038/332850a0
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发表时间：
1988-04-28
期刊：
影响因子：
64.8
通讯作者：
DEJEAN, A
中科院分区：
文献类型：
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作者：
BRAND, N;PETKOVICH, M;DEJEAN, A
我们以前已经描述了一个人的互补DNA，编码一种新的蛋白质，这是同源的类固醇/甲状腺核受体多基因家族的成员1。这种新的蛋白质（肝癌的hapfor）与人视黄酸受体（RAR）1，2具有很强的同源性，最近已被鉴定2，3。为了验证haptein也可能是一种维甲酸受体的可能性，我们用人雌激素受体（ER）的DNA结合域替换haptein的DNA结合域，从而产生了一种嵌合受体。然后测试所得hap-ER嵌合体在可能的受体配体存在下反式激活雌激素响应报告基因（vit-tk-CAT）的能力。在这里，我们表明，视黄酸（RA）在生理浓度是有效的，在诱导该报告基因的表达的hap-ER嵌合受体。这表明存在两种人视黄酸受体，命名为RAR-α和RAR-β。
We have previously described a human complementary DNA that encodes a novel protein which is homologous to members of the steroid/thyroid nuclear receptor multigene family1. This novel protein (hapfor hepatoma) exhibits strong homology with the human retinoic acid receptor (RAR)1,2which has been recently characterized2,3. To test the possibility that thehapprotein might also be a retinoid receptor, a chimaeric receptor was created by replacing the putative DNA binding domain ofhapwith that of the human oestrogen receptor (ER). The resultinghap-ER chimaera was then tested for its ability to trans-activate an oestrogen-responsive reporter gene (vit–tk–CAT) in the presence of possible receptor ligands. Here we show that retinoic acid (RA) at physiological concentrations is effective in inducing the expression of this reporter gene by thehap-ER chimaeric receptor. This demonstrates the existence of two human retinoic acid receptors designated RAR-α and RAR-β.