INFLUENCE OF SUGAR SOURCE (LACTOSE, GLUCOSE, GALACTOSE) ON 2,3-BUTANEDIOL PRODUCTION BY KLEBSIELLA-OXYTOCA NRRL-B199
INFLUENCE OF SUGAR SOURCE (LACTOSE, GLUCOSE, GALACTOSE) ON 2,3-BUTANEDIOL PRODUCTION BY KLEBSIELLA-OXYTOCA NRRL-B199
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DOI：
10.1007/bf00446920
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发表时间：
1989-10-01
影响因子：
2.8
通讯作者：
ROUXHET, PG
中科院分区：
文献类型：
--
作者：
CHAMPLUVIER, B;DECALLONNE, J;ROUXHET, PG
在不同通气和 pH 条件下的分批发酵中研究了产酸克雷伯菌 NRRL-B199 使用乳糖或葡萄糖和半乳糖混合物作为底物生产 2,3-丁二醇的能力。 2,3-丁二醇未检测到，或以微量浓度存在于含有乳糖的发酵液中，而它是葡萄糖 + 半乳糖的主要产物，在 pH 6.0 的培养基中最终浓度高达 18.8 g/l。在2,3-丁二醇合成的最佳条件下，当通气限制生长时，乳糖培养基中的生物量生长速率与通气速率的相关性比葡萄糖+半乳糖培养基中的生物量增长速率与通气速率的相关性更紧密。这些关系表明，当通气速率趋于零时，乳糖的生长速率非常低，但葡萄糖+半乳糖的生长速率仍然相当大。相应地，在前一种培养基中代谢更具氧化性，主要产生乙酸盐作为产物。
The ability of Klebsiella oxytoca NRRL-B199 to use either lactose or the mixture of glucose and galactose as substrate for the production of 2,3-butanediol was studied in batch fermentations with different conditions of aeration and pH. 2,3-butanediol was undetected, or present in minute concentration in the fermentation broths with lactose, while it was the main product from glucose + galactose with final concentrations of up to 18.8 g/l in media at pH 6.0. Under conditions optimal for 2,3-butanediol synthesis, when aeration limited growth, the rate of biomass growth was more tightly related to the aeration rate in lactose medium than in glucose + galactose medium. These relations suggest that the growth rate is very low on lactose but still considerable on glucose + galactose when aeration rate tends toward zero. Correspondingly, the metabolism is more oxidative in the former medium, yielding mainly acetate as product.