Early occurrence of human cytomegalovirus infection after bone marrow transplantation as demonstrated by the polymerase chain reaction technique.
Early occurrence of human cytomegalovirus infection after bone marrow transplantation as demonstrated by the polymerase chain reaction technique.
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聚合酶链反应技术证明骨髓移植后早期发生人巨细胞病毒感染。
DOI：
10.1182/blood.v77.5.1104.1104
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发表时间：
1991
期刊：
影响因子：
20.3
通讯作者：
C. Müller
中科院分区：
文献类型：
--
作者：
H. Einsele;M. Steidle;Angelika Vallbracht;Johannes G. Saal;G. Ehninger;C. Müller
使用聚合酶链式反应（PCR）、狭缝印迹杂交和常规病毒培养，对接受骨髓移植（BMT）的28名患者进行每周一次的随访，从第-10天到出院后针对人巨细胞病毒（HCMV）感染进行BMT。所应用的 PCR 测定的高特异性可以通过未能扩增从经常感染骨髓移植受者的多种其他病毒中提取的 DNA 来证明。 PCR 技术使我们能够在 BMT 后的 24 名血清阳性患者中诊断出 20 名（83%）患者存在病毒血症和病毒血症，而培养检测显示，其中 24 名患者中有 16 名（67%）患有病毒血症，24 例患者中有 9 名（37%）患有病毒血症。狭缝印迹杂交显示 24 名血清阳性患者中有 12 名（50%）出现病毒尿和病毒血症。通过应用PCR技术，即使在移植后的早期阶段也可以实现HCMV检测。甚至在急性移植物抗宿主病临床症状出现之前，就有 5 名患者检测到了 HCMV。对 BMT 后患者的 33 份器官活检标本进行 PCR 技术分析显示，从 HCMV 病毒血症患者获得的 14 份肝脏样本中，有 13 份存在 HCMV；采用所有技术，来自无病毒血症患者的三份肝脏标本均呈阴性。所有间质性肺炎患者 (n = 10) 的死后肺活检标本中也可证实 HCMV。
Twenty-eight patients undergoing bone marrow transplantation (BMT) were followed-up at weekly intervals from day -10 to discharge from hospital after BMT for human cytomegalovirus (HCMV) infection using polymerase chain reaction (PCR), slot-blot hybridization, and conventional virus culture. High specificity of the PCR assay applied could be shown by failure to amplify DNA extracted from a wide range of other viruses frequently infecting marrow transplant recipients. The PCR technique allowed us to diagnose viremia and viruria in 20 (83%) of 24 seropositive patients after BMT, whereas culture assays showed 16 (67%) of 24 of these patients to be viruric and 9 (37%) of 24 cases to be viremic. Slot-blot hybridization showed a frequency of viruria and viremia in 12 (50%) of 24 seropositive patients. By application of PCR techniques, HCMV detection could be achieved even in the very early posttransplant period. HCMV was detected in five patients even before the onset of clinical symptoms of acute graft-versus-host disease. Analysis by PCR techniques of 33 organ biopsy specimens from patients after BMT showed the presence of HCMV in 13 of 14 liver samples obtained from patients with HCMV viremia; three liver specimens from patients without viremia were negative by all the techniques applied. HCMV could also be demonstrated in postmortem lung biopsy specimens from all patients (n = 10) with interstitial pneumonia.
骨髓移植后巨细胞病毒感染：与急性移植物抗宿主病的关联。
影响因子：
20.3
作者：
Miller,W;Flynn,P;McCullough,J;BalfourJr,HH;Goldman,A;Haake,R;McGlave,P;Ramsay,N;Kersey,J
通讯作者：
Kersey,J