Separation of hepatic and gastrointestinal signals from the common "hepatic" branch of the vagus
Separation of hepatic and gastrointestinal signals from the common "hepatic" branch of the vagus
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DOI：
10.1152/ajpregu.00673.2003
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发表时间：
2004-07-01
影响因子：
2.8
通讯作者：
Friedman, MI
中科院分区：
文献类型：
--
作者：
Horn, CC;Friedman, MI
解剖学研究表明，迷走神经的肝总分支（CHB）含有传入纤维，支配肝门区以外的部位，主要是胃肠道。在目前的实验中，来自CHB的信号源是通过记录CHB的胃十二指肠分支（GDB）横断之前和之后的CHB神经生理反应来确定的。血清素[5-羟色胺（5-HT）]和CCK-8作为探针刺激CHB。大部分CHB传入纤维对5-HT敏感（56%），其中35%对CCK-8也敏感。门静脉与颈静脉灌注5-HT和CCK-8和GDB横断显示，5-HT和CCK敏感纤维支配肝门区和肝门外区域（例如，门静脉）。例如，在一个实施例中，胃肠道）。5-HT 3受体拮抗剂（Y-25130）对基底神经活动的抑制表明，大约50%的CHB传入纤维含有5-HT 3受体，但这些纤维似乎都不在肝门区，因为只有在GDB完整的大鼠中，Y-25130才降低神经活动。总之，这些数据与CHB纤维分布的解剖学观察结果非常一致，并表明CHB的神经生理学研究必须仔细评估，因为从CHB记录的非肝门静脉传入信号具有突出作用。
Anatomic studies show that the common hepatic branch (CHB) of the vagus contains afferent fibers that innervate sites outside the hepatoportal region, primarily in the gastrointestinal tract. In the current experiments on the anesthetized rat, the source of signals from the CHB was determined by recording CHB neurophysiological responses before and after transection of the gastroduodenal branch (GDB) of the CHB. Serotonin [5-hydroxytryptamine(5-HT)] and CCK-8 were used as probes to stimulate the CHB. Most of the CHB afferent fibers were 5-HT sensitive (56%), and 35% of these were also sensitive to CCK-8. Portal vein vs. jugular vein infusion of 5-HT and CCK-8 and GDB transection showed that 5-HT- and CCK-sensitive fibers innervate the hepatoportal region and areas outside the hepatic hilus ( e. g., the gastrointestinal tract). Suppression of basal nerve activity by a 5-HT3 receptor antagonist (Y-25130) suggests that similar to50% of CHB afferent fibers contain 5-HT3 receptors, but none of these fibers appears to be in the hepatoportal region because only in rats with an intact GDB did Y-25130 reduce nerve activity. In summary, these data are in close agreement with anatomic observations on the distribution of the CHB fibers and indicate that neurophysiological studies of the CHB must be carefully evaluated given the prominent role of nonhepatoportal afferent signals recorded from the CHB.