Adaptation of photoperiodic control pathways produces short-day flowering in rice
Adaptation of photoperiodic control pathways produces short-day flowering in rice
复制标题
DOI：
10.1038/nature01549
复制
发表时间：
2003-04-17
期刊：
影响因子：
64.8
通讯作者：
Shimamoto, K
中科院分区：
文献类型：
--
作者：
Hayama, R;Yokoi, S;Shimamoto, K
开花的光周期控制是植物重要的发育过程之一，因为它直接关系到成功的繁殖（1）。尽管拟南芥（Arabidopsis thaliana）（一种长日照（LD）植物）的分子遗传学分析提供了解释该物种中开花时间控制的模型（2-4），但关于其短日照（SD）植物的分子机制知之甚少。在这里，我们展示了如何在水稻，SD植物开花的光周期控制。OsGI（5）是拟南芥GIGANTEA（GI）基因（6，7）的直向同源物，在转基因水稻中过表达OsGI（5）在SD和LD条件下均导致开花延迟。在转基因水稻中，拟南芥CONSTANS（CO）基因（9）的水稻直向同源物（8）的表达增加，而FLOWERLOCUS T（FT）（11，12）的水稻直向同源物（10）的表达受到抑制。我们的研究结果表明，光周期控制开花的三个关键调控基因是保守的拟南芥，LD植物，和水稻，SD植物，但由CO调节FT基因被逆转，导致在LD条件下抑制水稻开花。
The photoperiodic control of flowering is one of the important developmental processes of plants because it is directly related to successful reproduction(1). Although the molecular genetic analysis of Arabidopsis thaliana, a long-day (LD) plant, has provided models to explain the control of flowering time in this species(2-4), very little is known about its molecular mechanisms for short-day (SD) plants. Here we show how the photoperiodic control of flowering is regulated in rice, a SD plant. Overexpression of OsGI(5), an orthologue of the Arabidopsis GIGANTEA (GI) gene(6,7) in transgenic rice, caused late flowering under both SD and LD conditions. Expression of the rice orthologue(8) of the Arabidopsis CONSTANS (CO) gene(9) was increased in the transgenic rice, whereas expression of the rice orthologue(10) of FLOWERING LOCUS T (FT)(11,12) was suppressed. Our results indicate that three key regulatory genes for the photoperiodic control of flowering are conserved between Arabidopsis, a LD plant, and rice, a SD plant, but regulation of the FT gene by CO was reversed, resulting in the suppression of flowering in rice under LD conditions.