Adhesin genes and serum resistance in Haemophilus influenzae type f isolates.
Adhesin genes and serum resistance in Haemophilus influenzae type f isolates.
复制标题
f 型流感嗜血杆菌分离株中的粘附素基因和血清抗性。
DOI：
10.1099/jmm.0.052175-0
复制
发表时间：
2013
影响因子：
3
通讯作者：
Smith,ArnoldL
中科院分区：
文献类型：
--
作者：
Watson,MichaelE;Nelson,KevinL;Nguyen,Victoria;Burnham,Carey-AnnD;Clarridge,JillE;Qin,Xuan;Smith,ArnoldL
The incidence of invasive infections due toHaemophilus influenzaehas decreased significantly in developed countries with high rates of vaccination againstH. influenzaeserotype b (Hib). This vaccine provides no protection againstH. influenzaeserotype f (Hif), typically associated with invasive infections in adults with chronic disease and/or immunodeficiency, and rarely in otherwise healthy adults and children. The specific properties of Hif associated with virulence remain largely uncharacterized. A panel of 26 Hif strains consisting of both invasive disease-associated and mucosal surface non-invasive disease-associated isolates was surveyed by DNA fingerprinting, biotyping and PCR detection ofhmw1,hmw2,hsf, thehiffimbrial locus and the lipo-oligosaccharide (LOS) biosynthetic island, and assessment of β-lactamase expression and determination of resistance to the bactericidal activity of normal adult human serum. Repetitive sequence-based PCR fingerprinting differentiated the 26 strains into three clusters, with the majority of isolates (22/26, 84.6 %) clustered into a single indistinguishable group. Most isolates (24/26, 92.3 %) were of biotype I and two isolates produced β-lactamase with detection of a conjugative plasmid, and the isolates displayed a range of resistances to the bactericidal activity of human serum. All 26 isolates carried the adhesinhsf, 21 carried a partialhiffimbrial operon and 4 had the adhesin geneshmw1/2.A LOS biosynthetic island was detected in 20 isolates consisting of the geneslic2BC. It was concluded that Hif has many recognized virulence properties and comprises a relatively homogeneous group independent of the anatomical source from which it was isolated.
