A study of the ultrastructural features of the cut margin of skin and mucous membrane specimens excised by carbon dioxide laser.
A study of the ultrastructural features of the cut margin of skin and mucous membrane specimens excised by carbon dioxide laser.
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二氧化碳激光切除皮肤粘膜标本切缘超微结构特征的研究。
DOI：
10.1016/0022-4804(76)90065-2
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发表时间：
1976
影响因子：
2.2
通讯作者：
I. Kaplan
中科院分区：
文献类型：
--
作者：
M. Ben;I. Kaplan
为了更精确地测量激光束的损伤深度，对三个皮肤和粘膜损伤的手术标本的切缘进行了电子显微镜检查，在光学显微镜下显示为“烧伤”边缘。这种材料的连续切片显示“烧伤”边缘延伸到200-250 μm的深度，但对所有组织的损伤并不相等。对激光束最敏感的是上皮细胞及其细胞器，其在距离切割边缘250 μm处显示出坏死迹象。桥粒、细胞核胶原纤维和红细胞对激光束的敏感性较低，并且在距外周30-50 μm深度处的超声切割上开始显示出其结构的一些保留。毛细血管造影显示内皮细胞肿胀，管腔明显狭窄。并对这些发现的重要性和激光在临床外科中的应用意义进行了讨论。
Electron microscopic examination was performed on the cut edge of three surgical specimens of skin and mucous membrane lesions to measure more precisely the depth of damage of the laser beams, appearing on light microscopy as “burn” rim.Serial sections of this material revealed that the “burn” rim extended to a depth of 200–250 μm, but damage was not equal for all tissue. Most sensitive to the laser beams are the epithelial cells and their organelles, which showed signs of necrosis to a distance of 250 μm from the cut edge. Desmosomes, cell nuclei collagen fibers, and erythrocytes are less sensitive to the laser beams and started showing some preservation of their structure on the ultrasections cut at a depth of 30–50 μm from the periphery. Capillaries revealed swollen endothelial cells with a marked narrowing of their lumen. The importance of these findings and the significance of the application of the laser in clinical surgery were discussed.