MicroRNA-873 acts as a tumor suppressor in esophageal cancer by inhibiting differentiated embryonic chondrocyte expressed gene 2
MicroRNA-873 acts as a tumor suppressor in esophageal cancer by inhibiting differentiated embryonic chondrocyte expressed gene 2
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DOI：
10.1016/j.biopha.2018.05.152
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发表时间：
2018-09-01
影响因子：
7.5
通讯作者：
Lu, Baolan
中科院分区：
文献类型：
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作者：
Liang, Yuqiang;Zhang, Peirong;Lu, Baolan
食道癌是世界范围内最常见的消化系统恶性肿瘤之一，越来越多的证据表明microRNAs(MiRNAs)与食道癌的发生发展密切相关。然而，microRNA-873(miR-873)在食道癌中的表达水平和生物学功能仍很难确定。在本研究中，我们研究了miR-873在食管癌中的表达及其生物学作用。我们的结果表明，miR-873在食管癌组织和细胞系中的表达明显低于癌旁组织和原代培养的人食道上皮细胞。此外，miR-873过表达可显著抑制食道癌细胞的生长、迁移和侵袭。此外，我们还验证了分化胚胎软骨细胞表达基因2(DEC2)是miR-873的直接靶点，可以逆转miR-873对食道癌细胞的抑制作用。综上所述，我们的研究表明miR-873在食道癌中低表达，并可能通过直接靶向DEC2而发挥肿瘤抑制基因的作用。
Esophageal cancer is one of the most common digestive malignant diseases worldwide and emerging evidences revealed that microRNAs (miRNAs) were implicated in the development and progression of esophageal cancer. However, the expression level and biological function of microRNA-873(miR-873) in esophageal cancer are still largely elusive. In this study, we investigated the expression and biological roles of miR-873 in human esophageal cancer. Our results revealed that miR-873 was significantly underexpressed in esophageal cancer tissues and cell lines when compared with the para-tumor tissue and primary human esophageal epithelial cells. Furthermore, overexpression of miR-873 could remarkably inhibit esophageal cancer cell growth, migration and invasion. Moreover, we validated differentiated embryonic chondrocyte expressed gene 2 (DEC2) as a direct target of miR-873 which could reverse the repressive effects of miR-873 on esophageal cancer cell. In summary, our investigation demonstrated that miR-873 was underexpressed in esophageal cancer and might act as a tumor suppressor gene by directly targeting DEC2.