Small RNA-Seq analysis reveals microRNA-regulation of the Imd pathway during Escherichia coli infection in Drosophila
Small RNA-Seq analysis reveals microRNA-regulation of the Imd pathway during Escherichia coli infection in Drosophila
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Small RNA-Seq 分析揭示果蝇大肠杆菌感染过程中 Imd 通路的 microRNA 调节
DOI：
10.1016/j.dci.2017.01.008
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发表时间：
2017-05-01
影响因子：
2.9
通讯作者：
Ma, Fei
中科院分区：
文献类型：
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作者：
Li, Shengjie;Shen, Li;Ma, Fei
几十年来，果蝇一直作为先天免疫研究的模型。然而，对于 microRNA (miRNA) 对免疫基因表达的转录后调控的了解仍然很初级。在本研究中，利用小RNA测序和生物信息学分析，我们在三个时间点鉴定了感染大肠杆菌的果蝇与受伤果蝇中67个差异表达的miRNA。此外，我们发现其中 21 个 miRNA 可能参与 Imd 通路相关基因的调控。引人注目的是，基于 UAS-miRNA 线筛选和双荧光素酶测定，我们发现 miR-9a 和 miR-981 都可以负向调节果蝇抗菌防御并降低抗菌肽双翅菌素的水平。总而言之，这些数据支持 miRNA 参与果蝇 Imd 通路的调节。 (C) 2017 Elsevier Ltd. 保留所有权利。
Drosophila have served as a model for research on innate immunity for decades. However, knowledge of the post-transcriptional regulation of immune gene expression by microRNAs (miRNAs) remains rudimentary. In the present study, using small RNA-seq and bioinformatics analysis, we identified 67 differentially expressed miRNAs in Drosophila infected with Escherichia coli compared to injured flies at three time-points. Furthermore, we found that 21 of these miRNAs were potentially involved in the regulation of Imd pathway-related genes. Strikingly, based on UAS-miRNAs line screening and Dualluciferase assay, we identified that miR-9a and miR-981 could both negatively regulate Drosophila antibacterial defenses and decrease the level of the antibacterial peptide, Diptericin. Taken together, these data support the involvement of miRNAs in the regulation of the Drosophila Imd pathway. (C) 2017 Elsevier Ltd. All rights reserved.