Paenibacillus shunpengii sp. nov., isolated from farmland soil.
Paenibacillus shunpengii sp. nov., isolated from farmland soil.
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DOI：
10.1099/ijsem.0.002484
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发表时间：
2018
影响因子：
2.8
通讯作者：
Youjian Yang;Yanting Zhang;Guo-Qiang Chen;Dan Cheng;J. Qiu;Qin He;Jian He
中科院分区：
文献类型：
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作者：
Youjian Yang;Yanting Zhang;Guo-Qiang Chen;Dan Cheng;J. Qiu;Qin He;Jian He
从我国农田土壤中分离到一株细菌，命名为YYJ7 - 1T，并对其进行了多相分类鉴定。菌株YYJ7 - 1T细胞革兰氏染色阳性，需氧或兼氧，杆状，能运动，能形成内孢子。生长发生在18 - 42 ° C（最佳35 ° C）、pH 6.0 - 8.0（最佳pH 7.5）和0.0 - 4.0%NaCl（最佳0.5%）下。基于16S rRNA基因序列的系统发育分析表明，该菌株属于类芽孢杆菌属（Paenibacillus），与普罗文森类芽孢杆菌4401170T（98.6%）和乌拉尔类芽孢杆菌5402403T（98.4%）具有较高的序列相似性，而与所有其他模式菌株的16S rRNA基因序列相似性较低（97.0%）。而YYJ7 - 1T菌株与P.provencensis4401170T48.7 ± 4.5%（43.6 ± 7.1%）和P.uraum5402403T38.9 ± 5.7%（35.6 ± 6.8%）的DNA-DNA相关性较低。菌株YYJ7 - 1T的主要脂肪酸（> 10.0%）为anteiso-C15：0、iso-C16：0和anteiso-C17：0。极性脂质谱由磷脂、二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺组成。主要的类异戊二烯醌是MK-7。DNA G + C含量为39.4mol%。根据这些结果，可以得出结论，菌株YYJ7 - 1T代表类芽孢杆菌属的一个新种，建议将其命名为顺盆类芽孢杆菌新种，模式菌株为YYJ7 - 1T（= ACCC 19965T = KCTC 33849T）。
A bacterial strain designated YYJ7-1T was isolated from farmland soil in China and characterized using a polyphasic taxonomic approach. Cells of strain YYJ7-1T were Gram-staining-positive, aerobic or facultatively anaerobic, rod-shaped, motile and endospore-forming. Growth occurred at 18-42 °C (optimum at 35 °C), at pH 6.0-8.0 (optimum at pH 7.5) and with 0.0-4.0 % NaCl (optimum with 0.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus Paenibacillus and showed high levels of sequence similarity with respect to Paenibacillus provencensis 4401170T (98.6 %) and Paenibacillus urinalis 5402403T (98.4 %), while lower 16S rRNA gene sequence similarities were observed with all other type strains (97.0 %). However, strain YYJ7-1T showed low DNA-DNA relatedness with P. provencensis 4401170T 48.7±4.5 % (43.6±7.1 % in a reciprocal experiment), and P. urinalis 5402403T 38.9±5.7 % (35.6±6.8 %). The major cellular fatty acids (>10.0 %) of strain YYJ7-1T were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The polar lipid profile consisted of phospholipids, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major isoprenoid quinone was MK-7. The DNA G+C content was 39.4 mol%. Based on these results, it is concluded that strain YYJ7-1T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus shunpengii sp. nov. is proposed, with YYJ7-1T (=ACCC 19965T=KCTC 33849T) as the type strain.