Stage-dependent differential gene expression profiles of cranial neural
Stage-dependent differential gene expression profiles of cranial neural
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颅神经阶段依赖性差异基因表达谱
DOI：
10.1007/s00795-019-00229-2
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Azuma T.
中科院分区：
文献类型：
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作者：
Odashima A;Onodera S;Saito A;Ogihara Y;Ichinohe T;Azuma T.
颅神经脊细胞是一种多能细胞，可迁移到脊椎动物胚胎的咽弓，并分化为各种头面部器官的衍生品。因此，移行的颅神经脊细胞被认为是再生医学中最具吸引力的候选细胞来源之一。我们用小鼠诱导的多能干细胞在神经脊诱导培养液中培养14天，获得了脑神经峰样细胞(CNCC)。随后，我们进行了RNA测序实验，分析了cNCC诱导后不同时间点的基因表达谱。CNCCs表达多个神经脊指定子基因，但已报道的胚胎神经脊发育所必需的配对盒3和叉头盒D3等指定基因不表达。此外，Ets原癌基因1、转录因子和性别决定区Y-box 10在诱导后14天才有表达。最后，cNCC表达多种原钙粘附素、去整合素和带有血栓反应蛋白基序的金属蛋白酶，这可能是其迁移的关键。
Cranial neural crest cells are multipotent cells that migrate into the pharyngeal arches of the vertebrate embryo and differentiate into various craniofacial organ derivatives. Therefore, migrating cranial neural crest cells are considered one of the most attractive candidate cell sources in regenerative medicine. We generated cranial neural crest like cell (cNCCs) using mouse-induced pluripotent stem cells cultured in neural crest-inducing medium for 14 days. Subsequently, we conducted RNA sequencing experiments to analyze gene expression profiles of cNCCs at different time points after induction. cNCCs expressed several neural crest specifier genes; however, some previously reported specifier genes such as paired box 3 and Forkhead box D3, which are essential for embryonic neural crest development, were not expressed. Moreover, ETS proto-oncogene 1, transcription factor and sex-determining region Y-box 10 were only expressed after 14 days of induction. Finally, cNCCs expressed multiple protocadherins and a disintegrin and metalloproteinase with thrombospondin motifs enzymes, which may be crucial for their migration.