Expression of Helios in Peripherally Induced Foxp3+ Regulatory T Cells
Expression of Helios in Peripherally Induced Foxp3+ Regulatory T Cells
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DOI：
10.4049/jimmunol.1102964
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发表时间：
2012-02-01
影响因子：
4.4
通讯作者：
Allison, James P.
中科院分区：
文献类型：
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作者：
Gottschalk, Rachel A.;Corse, Emily;Allison, James P.
据报道，转录因子Helios是胸腺来源的调节性T细胞(Treg)的标志，将它们与外周诱导的Treg区分开来。在这项研究中，我们证明了Helios在体内和体外都在Foxp3(+)iTreg中表达。跟随静脉注射。多肽注射后，体内过继转移的TCR转基因T细胞中Helios的表达比Foxp3诱导更快，但在以后的时间点不再注射多肽时稳定性较差。我们的体外数据表明，APC以不同于Foxp3诱导所需刺激的方式影响Helios的表达。因此，Helios在iTreg中的表达可能反映了Foxp3诱导过程中刺激的背景。总之，我们在iTreg中观察到的强大的Helios表达排除了它作为胸腺Treg的标记的可能性。免疫学杂志，2012,188：976-980。
The transcription factor Helios has been reported to be a marker of regulatory T cells (Treg) of thymic origin, distinguishing them from Treg induced in the periphery (iTreg). In this study, we demonstrate Helios expression in Foxp3(+) iTreg, both in vitro and in vivo. Following i.v. peptide injection, in vivo Helios expression in adoptively transferred TCR transgenic T cells was more rapid than Foxp3 induction but less stable at later time points without a second injection of peptide. Our in vitro data suggest that APC influence Helios expression in a manner distinct from stimuli required for Foxp3 induction. Thus, Helios expression in iTreg may reflect the context of stimulation during Foxp3 induction. In summary, the robust Helios expression we observe in iTreg precludes its use as a marker of thymic Treg. The Journal of Immunology, 2012, 188: 976-980.