Stimulation with phytohaemagglutinin induces the synthesis of catecholamines in human peripheral blood mononuclear cells: role of protein kinase C and contribution of intracellular calcium
Stimulation with phytohaemagglutinin induces the synthesis of catecholamines in human peripheral blood mononuclear cells: role of protein kinase C and contribution of intracellular calcium
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DOI：
10.1016/s0165-5728(02)00019-x
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发表时间：
2002-04-01
影响因子：
3.3
通讯作者：
Frigo, G
中科院分区：
文献类型：
--
作者：
Cosentino, M;Marino, F;Frigo, G
虽然现在已经确定，免疫活性细胞产生的儿茶酚胺（CA），这反过来又可能作为自分泌/旁分泌介质，很少有人知道的机制，调节CA生产这些细胞。在本研究中，提供的证据表明，刺激人培养的外周血单核细胞（PBMC）与植物血凝素（PHA）诱导酪氨酸羟化酶（TH）mRNA的表达，随后增加细胞内CA水平通过蛋白激酶C（PKC）激活和细胞内Ca 2+依赖性机制的贡献。PHA刺激的PBMC中CA的产生增加表明在活化细胞的功能调节中优先参与儿茶酚胺能途径。这些发现可能有助于更好地定义免疫活性细胞来源的CA在神经免疫网络中的作用。(C)2002 Elsevier Science B. V.保留所有权利。
Although it is now established that immunocompetent cells produce catecholamines (CA), which in turn may act as autocrine/paracrine mediators, little is known about the mechanisms regulating CA production in these cells. In the present study, evidence is provided that stimulation of human Cultured peripheral blood mononuclear cells (PBMCs) with phytohaemagglutinin (PHA) induces the expression of tyrosine hydroxylase (TH) mRNA and subsequently increases intracellular CA levels through protein kinase C (PKC) activation and the contribution of intracellular Ca2+-dependent mechanisms. Increased production of CA in PHA-stimulated PBMCs suggests a preferential involvement of catecholaminergic pathways in the functional modulation of activated cells. These findings may help to better define the role of immunocompetent cell-derived CA in the neuroimmune network. (C) 2002 Elsevier Science B.V. All rights reserved.