Proxy comparison in ancient peat sediments: pollen, macrofossil and plant DNA
Proxy comparison in ancient peat sediments: pollen, macrofossil and plant DNA
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DOI：
10.1098/rstb.2013.0382
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发表时间：
2015-01-19
影响因子：
6.3
通讯作者：
Suyama, Yoshihisa
中科院分区：
文献类型：
--
作者：
Parducci, Laura;Valiranta, Minna;Suyama, Yoshihisa
我们比较了从北欧 Weichselian interstadial（年龄超过 40 kyr）和全新世（最大年龄 8400 cal yr BP）泥炭沉积物中获得的 DNA、花粉和大化石数据，并用它们重建了两个地点的当代植物区系组成。大多数样本提供了高质量的植物 DNA 序列，成功扩增率取决于年龄。 DNA 和测序分析提供了来自较旧站点的 5 个植物类群和来自较新站点的 9 个植物类群，分别相当于三个代理识别的类群总数的 7% 和 15%。在这两个地点，花粉分析检测到最多（54）个类群，DNA 最少（10）个类群，但花粉和大型化石未检测到 5 个 DNA 类群。 DNA 和花粉之间的重叠比 DNA 和大化石代理之间更大的重叠的发现似乎与我们之前基于湖泊沉积物的建议相悖，即 DNA 主要来源于植物组织，较少来自花粉。在这两个地点，我们还检测到了栎属。 DNA，但记录中几乎没有发现花粉粒，这些通常被解释为长距离传播。我们证实，在古生态学研究中，沉积 DNA 分析不如经典形态学分析全面，但它是获得过去植物区系更完整图景的补充且重要的工具。
We compared DNA, pollen and macrofossil data obtained from Weichselian interstadial (age more than 40 kyr) and Holocene (maximum age 8400 cal yr BP) peat sediments from northern Europe and used them to reconstruct contemporary floristic compositions at two sites. The majority of the samples provided plant DNA sequences of good quality with success amplification rates depending on age. DNA and sequencing analysis provided five plant taxa from the older site and nine taxa from the younger site, corresponding to 7% and 15% of the total number of taxa identified by the three proxies together. At both sites, pollen analysis detected the largest (54) and DNA the lowest (10) number of taxa, but five of the DNA taxa were not detected by pollen and macrofossils. The finding of a larger overlap between DNA and pollen than between DNA and macrofossils proxies seems to go against our previous suggestion based on lacustrine sediments that DNA originates principally from plant tissues and less from pollen. At both sites, we also detected Quercus spp. DNA, but few pollen grains were found in the record, and these are normally interpreted as long-distance dispersal. We confirm that in palaeoecological investigations, sedimentary DNA analysis is less comprehensive than classical morphological analysis, but is a complementary and important tool to obtain a more complete picture of past flora.