Grb2 overexpression in nuclei and cytoplasm of human breast cells: a histochemical and biochemical study of normal and neoplastic mammary tissue specimens
Grb2 overexpression in nuclei and cytoplasm of human breast cells: a histochemical and biochemical study of normal and neoplastic mammary tissue specimens
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DOI：
10.1002/(sici)1096-9896(199710)183:2
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发表时间：
1997-10
期刊：
影响因子：
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通讯作者：
Bianca S. Verbeek;S. Adriaansen‐slot;G. Rijksen;T. Vroom
中科院分区：
文献类型：
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作者：
Bianca S. Verbeek;S. Adriaansen‐slot;G. Rijksen;T. Vroom
在20- 30%的人类乳腺癌中，受体酪氨酸激酶表皮生长因子受体（EGFR）和c-erbB 2过表达。这种过度表达导致促有丝分裂信号传导增加，并且与不良预后相关。相关衔接蛋白（如Grb 2）的过表达也可诱导信号通路的上调。在这项研究中，使用免疫印迹实验和石蜡包埋组织切片上的免疫染色，确定了Grb 2衔接蛋白在正常人乳腺组织和乳腺癌中的表达。生化和免疫组化技术均显示Grb 2在所有乳腺癌标本中过表达。此外，尽管Grb 2蛋白被描述为定位于细胞质中，但它也可以在正常和肿瘤乳腺组织中的细胞核中检测到。在乳腺肿瘤组织中，58%的Grb 2蛋白存在于细胞核中，而37%存在于细胞质中。在正常乳腺组织中，22%的Grb 2存在于细胞核中，70%存在于细胞质中。这些发现表明，在人类乳腺癌中，Grb 2过表达，似乎主要定位于细胞核。© 1997 John Wiley & Sons，Ltd.
In 20–30 per cent of human breast cancers, the receptor tyrosine kinases epidermal growth factor receptor (EGFR) and c‐erbB2 are overexpressed. This overexpression leads to increased mitogenic signalling and is correlated with poor prognosis. Overexpression of associated adaptor proteins, like Grb2, can also induce upregulation of signalling pathways. In this study, the expression of the Grb2 adaptor protein was determined in both normal human breast tissue and mammary cancers, using immunoblotting experiments and immunostaining on paraffin‐embedded tissue sections. Both biochemical and immunohistochemical techniques revealed overexpression of Grb2 in all breast cancer specimens. In addition, although Grb2 protein is described as localized in the cytoplasm, it can also be detected in the nucleus, both in normal and in tumour breast tissue. In tumour breast tissue, 58 per cent of Grb2 protein is found in the nucleus, while 37 per cent is detected in the cytoplasm. In normal breast tissue, 22 per cent of Grb2 is found in the nucleus and 70 per cent in the cytoplasm. These findings indicate that in human breast cancer, Grb2 is overexpressed and appears to be predominantly localized in the nucleus. © 1997 John Wiley & Sons, Ltd.