Measurement of isocitric dehydrogenase activity in body fluids.
Measurement of isocitric dehydrogenase activity in body fluids.
复制标题
体液中异柠檬酸脱氢酶活性的测量。
作者：
G. Bowers
1.本文研究了血清异柠檬酸脱氢酶（ICD）活性的影响因素。观察到高浓度异柠檬酸盐的底物抑制。
2.测定血清ICD的温度-活性曲线。强调了控制反应温度的重要性。
3.使用该信息，测量0.2 ml中ICD活性的方法。血清的描述。
4. 50例健康男性ICD活性范围为30 ~ 192单位，平均90单位，标准差为± 34.3单位。
1. Factors influencing the isocitric dehydrogenase (ICD) activity of serum were studied. Substrate inhibition by high concentrations of isocitrate was observed.
2. The temperature-activity curve of serum ICD was determined. The importance of controlling the temperature of the reaction was stressed.
3. Using this information, a method of measuring the ICD activity in 0.2 ml. of serum is described.
4. The range of ICD activity in 50 healthy males was 30 to 192 units with a mean of 90 units and a standard deviation of ± 34.3 units.