EFFECT OF THE DEGREE OF MATURATION OF MOUSE OOCYTES AT FERTILIZATION - A SOURCE OF CHROMOSOME IMBALANCE
EFFECT OF THE DEGREE OF MATURATION OF MOUSE OOCYTES AT FERTILIZATION - A SOURCE OF CHROMOSOME IMBALANCE
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DOI：
10.1002/mrd.1120240208
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发表时间：
1989-10-01
期刊：
影响因子：
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通讯作者：
EGOZCUE, J
中科院分区：
文献类型：
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作者：
BADENAS, J;SANTALO, J;EGOZCUE, J
体外受精过程中人卵母细胞异常成熟可能导致染色体失衡，诱发胚胎丢失。利用小鼠模型，我们研究了卵母细胞成熟程度（不成熟或过成熟）对胚胎第一次卵裂分裂时染色体特征的影响。在预期排卵时间（b.o.）前2-3 h或3-4 h获得未成熟卵母细胞。新排卵的卵母细胞在体外老化3、6和12 h诱导过成熟。我们的研究结果表明，未成熟组（2-3 h为65.53%，3-4 h为16.59%，对照组为78.22%）和体外老化12 h后受精率显著降低（69.39%），而在体外老化3 h时该参数显著升高（82.59%），与其他组相比。未成熟、新排卵和过成熟卵母细胞获得的胚胎的非整倍体和超单倍体发生率无显著差异。最后，与对照组（14.59%）相比，未成熟组（2-3 h）（31.20%）和过成熟组（3 h）（23.04%）和6 h（31.61%）的多倍体发生率均有所增加。
It has been suggested that the abnormal maturation of the human oocyte during fertilization in vitro may result in chromosome imbalance and induce embryonic loss. Using a mouse model, we have studied the influence of the degree of oocyte maturation (either immaturity or overmaturity) on the chromosome characteristics of embryos at the first-cleavage division. Immature oocytes were obtained 2-3 h or 3-4 h before the expected ovulation time (b.o.). Overmaturation was induced by aging the newly ovulated oocytes in vitro for 3, 6, and 12 h. Our results show a significant decrease in the fertilization rate in the immature groups (65.53% at 2-3 h b.o. and 16.59% at 3-4 b.o. vs. 78.22% at control) and after 12 h of in vitro aging (69.39%), while a significant increase of this parameter was found at 3 h of aging (82.59%) as compared to the other groups. No significant differences were found in the occurrence of aneuploidy and hyperhaploidy in embryos obtained from immature, newly ovulated, and overmature oocytes. Finally, an increased incidence of polyploidy was detected in immature, 2-3 h b.o. (31.20%), and overmature, 3 h (23.04%) and 6 h (31.61%), groups as compared to the control group (14.59%).