A 45‐bp proximal region containing AACA and GCN4 motif is sufficient to confer endosperm‐specific expression of the rice storage protein glutelin gene, GluA‐3
A 45‐bp proximal region containing AACA and GCN4 motif is sufficient to confer endosperm‐specific expression of the rice storage protein glutelin gene, GluA‐3
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DOI：
10.1016/0014-5793(96)00233-5
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发表时间：
1996-04
期刊：
影响因子：
3.5
通讯作者：
T. Yoshihara;H. Washida;F. Takaiwa
中科院分区：
文献类型：
--
作者：
T. Yoshihara;H. Washida;F. Takaiwa
水稻谷蛋白启动子 (−104/−60) 的 45 bp 近端区域包含两个假定的顺式元件，即 AACA 基序和 GCN4 基序，与截短的 CaMV 35S 启动子 (−90/+9; -90Δ35S)/GUS 融合。 45 bp 片段特异性增强了转化烟草胚乳组织中的启动子活性。 GCN4 基序的取代突变降低了启动子活性，而 AACA 基序的突变则增加了胚胎和胚乳中的活性。这些结果表明 GCN4 基序通常增强启动子活性，但两个基序的组合赋予胚乳特异性。此外，两个基序的功能取决于-90Δ35S中G-box元件的方向和/或距离，这表明识别这些基序的因子和G-box元件之间的协同相互作用对于转录调控很重要。
A 45‐bp proximal region of the rice glutelin promoter (−104/−60) containing two putativecis‐elements, the AACA motif and GCN4 motifs, was fused to a truncated CaMV 35S promoter (−90/+9; −90Δ35S)/GUS. The 45‐bp fragment specifically enhanced the promoter activity in endosperm tissue of transformed tobacco. A substitution mutation of the GCN4 motif reduced the promoter activity, whereas mutation of the AACA motif increased the activity in the embryo as well as in the endosperm. These results suggest that the GCN4 motif generally enhances the promoter activity but that the combination of the two motifs confers the endosperm specificity. Furthermore, the function of the two motifs was dependent on the orientation and/or distance from a G‐box element in −90Δ35S, suggesting that synergistic interaction between the factors that recognize those motifs and the G‐box element is important for transcriptional regulation.