Cotranslational Intersection between the SRP and GET Targeting Pathways to the Endoplasmic Reticulum of Saccharomyces cerevisiae
Cotranslational Intersection between the SRP and GET Targeting Pathways to the Endoplasmic Reticulum of Saccharomyces cerevisiae
复制标题
SRP 和 GET 靶向酿酒酵母内质网途径之间的共翻译交叉
DOI：
10.1128/mcb.00131-16
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发表时间：
2016
影响因子：
5.3
通讯作者：
Rospert
中科院分区：
文献类型：
--
作者：
Y. Wölfle;Fitzke;Rospert
跨膜蛋白靶向内质网（ER）通过信号识别颗粒（SRP）或引导进入尾锚定蛋白（GET）途径进行，由Get 1至-5和Sgt 2组成。SRP协同靶向含有一个或多个跨膜结构域的膜蛋白，而GET途径协同靶向含有称为尾锚的单个C-末端跨膜结构域的蛋白。在这里，我们剖析的SRP和GET途径的同源，两个跨膜K+通道蛋白称为KCV，Kesv和Kesv-VV的排序中的作用。我们表明Kcv通过其N末端跨膜结构域共翻译靶向ER，而Kesv-VV通过其C末端跨膜结构域翻译后靶向ER，该结构域招募Get 4 -5/Sgt 2和Get 3。出乎意料的是，新生的Kcv不仅招募SRP，而且还将GET途径的Get 4 -5模块招募到核糖体。Get 4 -5的核糖体结合不依赖于Sgt 2，并且被SRP强烈地竞争。合并的数据表明，以前未认识到的共同翻译之间的相互作用的SRP和GET途径。
Targeting of transmembrane proteins to the endoplasmic reticulum (ER) proceeds via either the signal recognition particle (SRP) or the guided entry of tail-anchored proteins (GET) pathway, consisting of Get1 to -5 and Sgt2. While SRP cotranslationally targets membrane proteins containing one or multiple transmembrane domains, the GET pathway posttranslationally targets proteins containing a single C-terminal transmembrane domain termed the tail anchor. Here, we dissect the roles of the SRP and GET pathways in the sorting of homologous, two-membrane-spanning K+channel proteins termed Kcv, Kesv, and Kesv-VV. We show that Kcv is targeted to the ER cotranslationally via its N-terminal transmembrane domain, while Kesv-VV is targeted posttranslationally via its C-terminal transmembrane domain, which recruits Get4-5/Sgt2 and Get3. Unexpectedly, nascent Kcv recruited not only SRP but also the Get4-5 module of the GET pathway to ribosomes. Ribosome binding of Get4-5 was independent of Sgt2 and was strongly outcompeted by SRP. The combined data indicate a previously unrecognized cotranslational interplay between the SRP and GET pathways.
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DOI：
10.1073/pnas.1006036107
发表时间：
2010-07-06
影响因子：
11.1
作者：
Chartron, Justin W.;Suloway, Christian J. M.;Clemons, William M., Jr.
通讯作者：
Clemons, William M., Jr.
影响因子：
4
作者：
Kemper, Christian;Habib, Shukry J.;Rapaport, Doron
通讯作者：
Rapaport, Doron
体外蛋白质跨酿酒酵母微粒体膜易位。
影响因子：
--
作者：
P. Garcia;W. Hansen;P. Walter
通讯作者：
P. Walter
影响因子：
3.3
作者：
Zhang Y;Berndt U;Gölz H;Tais A;Oellerer S;Wölfle T;Fitzke E;Rospert S
通讯作者：
Rospert S
影响因子：
4.8
作者：
Chang, Yi-Wei;Chuang, Yi-Chien;Wang, Chung
通讯作者：
Wang, Chung