CELL-TO-SUBSTRATUM ADHESION AND GUIDANCE OF AXONAL ELONGATION
CELL-TO-SUBSTRATUM ADHESION AND GUIDANCE OF AXONAL ELONGATION
复制标题
DOI：
10.1016/0012-1606(75)90379-6
复制
发表时间：
1975-01-01
影响因子：
2.7
通讯作者：
LETOURNEAU, PC
中科院分区：
文献类型：
--
作者：
LETOURNEAU, PC
观察了轴突生长锥在基质中有图案变化的表面上的行为。将8日龄鸡胚感觉神经节细胞分别培养在塑料培养皿、塑料组织培养皿和聚氮氨酸包被组织培养皿上，培养皿中均含有钯沉积的网格状图案。结果表明，生长锥在Pd阴影区和缺乏Pd沉积区的伸长取决于生长锥对基质的相对粘附性。在培养皿中，生长锥停留在Pd上；在组织培养皿中，它们从一个表面交叉到另一个表面；而在聚氨酸涂层的盘子中，它们在无pd区域上拉长了很远的距离。延时电影分析表明，在Pd阴影下的多鸟氨酸培养皿上，生长锥经常接近Pd涂层区域，微尖接触Pd表面。然而，轴突尖端在无pd的多鸟氨酸表面继续伸长。结果表明，微穗与生长锥附近基质的相互作用是轴突伸长方向和途径的重要决定因素。
The behavior of axonal growth cones on surfaces with patterned variations in substratum was observed. Cells from sensory ganglia of 8-day-old chicken embryos were cultured on plastic petri dishes, plastic tissue culture dishes, and polyornithine-coated tissue culture dishes, all of which contained gridlike patterns of palladium (Pd) deposition.The results indicated that growth cones elongated on the Pd-shadowed areas vs areas lacking Pd deposits depending on the relative adhesivity of the growth cones to the substrata. In petri dishes, growth cones stay on the Pd; in tissue culture dishes, they cross from one surface to the other; and in polyornithine-coated dishes, they elongate for great distances on the Pd-free areas. Analyses of time-lapse movies showed that, on Pd-shadowed polyornithine dishes, growth cones often approach the Pd-coated areas and microspikes touch the Pd surface. Yet, the axon tip continues to elongate on the Pd-free polyornithine surface.The conclusion is offered that interactions between microspikes and the substratum adjacent to the growth cone are important determinants of the directions and pathways of axonal elongation.