Attenuation of exercise-induced heat shock protein 72 expression blunts improvements in whole-body insulin resistance in rats with type 2 diabetes
Attenuation of exercise-induced heat shock protein 72 expression blunts improvements in whole-body insulin resistance in rats with type 2 diabetes
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DOI：
10.1007/s12192-017-0767-z
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发表时间：
2017-03-01
影响因子：
3.8
通讯作者：
Naito, Hisashi
中科院分区：
文献类型：
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作者：
Tsuzuki, Takamasa;Kobayashi, Hiroyuki;Naito, Hisashi
热休克蛋白（HSP）在胰岛素抵抗中起重要作用，通过运动诱导HSP改善细胞应激反应治疗2型糖尿病。本研究探讨运动诱导的热休克蛋白72表达水平对2型糖尿病大鼠全身胰岛素抵抗的影响。将25周龄的雄性大冢长埃文斯德岛肥胖大鼠分为三组：久坐组（Sed）、在热中性环境中训练组（NTr：25 ℃）和在冷环境中训练组（CTr：4 ℃）。运动训练每周5天，共10周。每次运动后测量直肠温度。训练后进行腹膜内葡萄糖耐量试验（IPGTT）。在最后一次运动后48 h采集血清、腓肠肌和肝脏样品。Western blot检测热休克蛋白72和热休克蛋白73的表达水平，并检测血清总胆固醇、甘油三酯（TG）和游离脂肪酸（FFA）水平。NTr动物在运动后表现出显着较高的体温，而CTr动物则没有。运动训练可使腓肠肌和肝脏中HSP 72的表达增加，而CTr组HSP 72的表达显著低于NTr组（p < 0.05）。在两种训练的动物中，葡萄糖耐量同等地改善;然而，在IPGTT期间，CTr动物中的胰岛素水平高于NTr动物中的胰岛素水平（p < 0.05）。此外，与Sed动物相比，仅NTr动物的TG和FFA水平显著降低。这些结果表明，运动诱导的HSP 72表达的衰减部分钝化改善全身胰岛素抵抗和2型糖尿病大鼠的脂质代谢。
Heat shock proteins (HSPs) play an important role in insulin resistance and improve the cellular stress response via HSP induction by exercise to treat type 2 diabetes. In this study, the effects of exercise-induced HSP72 expression levels on whole-body insulin resistance in type 2 diabetic rats were investigated. Male 25-week-old Otsuka Long-Evans Tokushima Fatty rats were divided into three groups: sedentary (Sed), trained in a thermal-neutral environment (NTr: 25 degrees C), and trained in a cold environment (CTr: 4 degrees C). Exercise training was conducted 5 days/week for 10 weeks. Rectal temperature was measured following each bout of exercise. An intraperitoneal glucose tolerance test (IPGTT) was performed after the training sessions. The serum, gastrocnemius muscle, and liver were sampled 48 h after the final exercise session. HSP72 and heat shock cognate protein 73 expression levels were analyzed by Western blot, and serum total cholesterol, triglyceride (TG), and free fatty acid (FFA) levels were measured. NTr animals exhibited significantly higher body temperatures following exercise, whereas, CTr animals did not. Exercise training increased HSP72 levels in the gastrocnemius muscle and liver, whereas, HSP72 expression was significantly lower in the CTr group than that in the NTr group (p < 0.05). Glucose tolerance improved equally in both trained animals; however, insulin levels during the IPGTT were higher in CTr animals than those in NTr animals (p < 0.05). In addition, the TG and FFA levels decreased significantly only in NTr animals compared with those in Sed animals. These results suggest that attenuation of exercise-induced HSP72 expression partially blunts improvement in whole body insulin resistance and lipid metabolism in type 2 diabetic rats.