Interchromosornal interactions and olfactory receptor choice
Interchromosornal interactions and olfactory receptor choice
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DOI：
10.1016/j.cell.2006.06.035
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发表时间：
2006-07-28
期刊：
影响因子：
64.5
通讯作者：
Axel, Richard
中科院分区：
文献类型：
--
作者：
Lomvardas, Stavros;Barnea, Gilad;Axel, Richard
单个气味受体（odorant receptor，OR）基因在单个感觉神经元中的表达是嗅觉系统组织和功能的重要特征。我们已经使用染色体构象捕获来证明14号染色体上的增强子元件H与不同染色体上的多个OR基因启动子的特异性关联。DNA和RNA荧光原位杂交（FISH）实验使我们能够可视化的H增强子与单一的OR等位基因，这是在感觉神经元中转录的共定位。在携带额外的H元件的转基因小鼠中，表达OR假基因的感觉神经元也表达第二种功能性受体。这些数据表明，一个模型的受体选择，其中一个单一的反式作用增强子元件可能允许随机激活的嗅觉感觉神经元中只有一个OR等位基因。
The expression of a single odorant receptor (OR) gene from a large gene family in individual sensory neurons is an essential feature of the organization and function of the olfactory system. We have used chromosome conformation capture to demonstrate the specific association of an enhancer element, H, on chromosome 14 with multiple OR gene promoters on different chromosomes. DNA and RNA fluorescence in situ hybridization (FISH) experiments allow us to visualize the colocalization of the H enhancer with the single OR allele, that is transcribed in a sensory neuron. In transgenic mice bearing additional H elements, sensory neurons that express OR pseudogenes also express a second functional receptor. These data suggest a model of receptor choice in which a single trans-acting enhancer element may allow the stochastic activation of only one OR allele in an olfactory sensory neuron.