[Microscopic studies of Streptomyces hygroscopicus growth kinetics].
[Microscopic studies of Streptomyces hygroscopicus growth kinetics].
复制标题
吸水链霉菌生长动力学的显微研究。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
F. Bergter
中科院分区：
文献类型：
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作者：
E. Schuhmann†;F. Bergter
采用微培养的方法，用相差显微镜研究了吸水链霉菌在固体培养基上的生长动力学、分支形成和菌丝生长的细胞学特性。对从孢子萌发开始到最长18小时的生长菌丝的照片进行测量。芽管的比生长速率远高于菌丝体的比生长速率。菌丝总长度的倍增时间和分支形成的倍增时间在所研究的时期内是完全相同的。在短时间的生长之后，每个单独的菌丝以恒定速率生长，即长度线性增加，但是整个菌丝体的生长动力学通过分支而呈指数增长。看来，类核只在菌丝的顶端和最近的分支之间的那一部分分裂。一个细胞单位（1.4-1.9妈妈在不同的媒体）可以计算出菌丝的长度和核的数量。菌丝以顶端的细胞单位或仅以极帽生长。在复合培养基上，从顶端到约100 μ m处才形成分枝，在无机盐培养基上，从顶端到约50 μ m处才形成分枝。一种简单的菌丝生长方法已经开发出来。讨论了链霉菌与原核生物和菌丝生长真菌之间的联系。
Growth kinetics, branch formation, and cytological properties of mycelial growth of Streptomyces hygroscopicus on solid media were investigated by phase-contrast microscopy using a microculture method. Measurements were made on taken photographs of the growing hyphae from the beginning of spore germination up to maximal 18 hours. The specific growth rate of the germ tube was much higher than the specific growth rate of the mycelium. The doubling time of the total length of the mycelial hyphae and the doubling time of branch formation was quite the same for the period investigated. After a short time of outgrowth each individual hypha grows at a constant rate, i.e. the length increases linearly, but the growth kinetics of the whole mycelium becomes exponentially by branching. It seems, that nucleoids only divide in that part of a hypha between the tip and the nearest branch. A cell unit (1.4-1.9 mum on different media) could be calculated by the length of a hypha and the number of nucleoids. A hypha is growing with the cell unit at the tip or with the polar cap only. No interkalary growth in length could be found. Branches were formed only up to about 100 mum from the tip on complex medium and up to about 50 mum from the tip on mineral salt medium. A simple method of mycelial growth has been developed. Some properties showing the connection between Streptomycetes and prokaryotic organisms on one hand and hyphal growing fungi on the other hand are discussed.