The genomic organization of the gene encoding the vanilloid receptor: evidence for multiple splice variants.
The genomic organization of the gene encoding the vanilloid receptor: evidence for multiple splice variants.
复制标题
编码香草酸受体的基因的基因组组织：多种剪接变体的证据。
DOI：
10.1006/geno.2001.6582
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Schumacher,MA
中科院分区：
文献类型：
--
作者：
Xue,Q;Yu,Y;Trilk,SL;Jong,BE;Schumacher,MA
香草酸受体亚型 1 (VR1) 是一种非选择性阳离子通道，在感觉神经元中表达，并被多种有害刺激激活。大鼠 Vr1、拉伸失活通道 (SIC) 和香草酸受体 5' 剪接变体 (VR.5'sv) 被假设源自共同的 VR 基因。编码大鼠Vr1 5'部分的基因组结构的表征证实VR.5'sv源自VR基因；然而，SIC 似乎源自两个相关但独立的基因。我们还推导出了人类基因 VR1 的基因组结构。对大鼠和人类 VR 基因的比较研究表明，基因组组织具有显着的保守性。大鼠和人类 VR1 中外显子-内含子 7 侧翼的剪接位点与预期的共有序列不同；这可能有助于解释 VR.5'sv 和其他 VR 剪接变体中外显子 7 的跳跃。
Vanilloid receptor subtype-1 (VR1) is a nonselective cation channel that is expressed in sensory neurons and is activated by multiple noxious stimuli. Rat Vr1, stretch-inactivated channel (SIC), and vanilloid receptor 5′ splice variant (VR.5′sv) have been hypothesized to be derived from a common VR gene. Characterization of the genomic structure encoding the 5′ portion of rat Vr1 confirmed that VR.5′sv is derived from the VR gene; however, SIC seemed to be derived from two related but independent genes. We also deduced the genomic organization of the human gene VR1. Comparative studies of rat and human VR genes showed substantial conservation in genomic organization. The splice site flanking exon–intron 7 in rat and human VR1 diverged from the expected consensus sequence; this may help to explain the skipping of exon 7 within VR.5′sv and other VR splice variants.