Amphibian skin secretomics:: Application of parallel quadrupole time-of-flight mass spectrometry and peptide precursor cDNA cloning to rapidly characterize the skin secretory peptidome of Phyllomedusa hypochondrialis azurea:: Discovery of a novel peptide family, the hyposins
Amphibian skin secretomics:: Application of parallel quadrupole time-of-flight mass spectrometry and peptide precursor cDNA cloning to rapidly characterize the skin secretory peptidome of Phyllomedusa hypochondrialis azurea:: Discovery of a novel peptide family, the hyposins
复制标题
DOI：
10.1021/pr0702666
复制
发表时间：
2007-09-01
影响因子：
4.4
通讯作者：
McClean, Stephen
中科院分区：
文献类型：
--
作者：
Thompson, Alan Hunter;Bjourson, Anthony John;McClean, Stephen
本研究报道了蓝腹叶蛙（Phyllomedusa hypochondrialis azurea）皮肤分泌肽组中存在的多种肽。通过四极杆飞行时间串联质谱（QTOF MS/MS）分析、cDNA测序或两者结合的方法，阐明了肽的结构以及翻译后修饰。从六个不同的结构类别中鉴定出22种肽，包括19种新结构，这些类别包括色氨肽、皮啡肽以及一组被称为蓝腹肽（hyposins）的新型肽。该研究证明了这种联合方法在挖掘两栖动物防御性皮肤分泌物丰富肽组方面的强大作用。
This study reports the variety of peptides present in the skin secretory peptidome of Phyllomedusa hypochondrialis azurea. Peptide structures, along with post-translational modifications, were elucidated by QTOF MS/MS analysis, cDNA sequencing, or a combination of both. Twenty-two peptides, including 19 novel structures, were identified from six different structural classes, including tryptophyllins, dermorphins, and a novel group of peptides termed hyposins. The study demonstrates the power of this combined approach to mine the rich peptidome compliment of the amphibian defensive skin secretome.