Conjugated polyelectrolytes:: Conformation-sensitive optical probes for detection of arnyloid fibril forrnation
Conjugated polyelectrolytes:: Conformation-sensitive optical probes for detection of arnyloid fibril forrnation
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DOI：
10.1021/bi047402u
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发表时间：
2005-03-15
期刊：
影响因子：
2.9
通讯作者：
Inganäs, O
中科院分区：
文献类型：
--
作者：
Nilsson, KPR;Herland, A;Inganäs, O
淀粉样蛋白原纤维的体内沉积是许多被称为淀粉样病变的破坏性疾病的标志。体外淀粉样蛋白的形成也可能使生物技术工业中蛋白质的生产复杂化。因此，检测淀粉样蛋白原纤维构象的简单、灵敏和通用的工具是非常重要的。我们已经开发了一种带负电荷的共轭聚电解质，根据与之形成复合物的蛋白质是处于天然状态还是淀粉样纤维构象，它可以通过视觉或吸收和发射检测到不同的光学变化特征。本文将这种简单、快速、新颖的方法应用于胰岛素和溶菌酶两种淀粉样蛋白，并通过圆二色性、透射电子显微镜和刚果红吸收等目前使用的方法验证了其检测纤维构象的有效性。
The in vivo deposition of amyloid fibrils is a hallmark of many devastating diseases known n as the amyloidoses. Amyloid formation in vitro may also complicate production of proteins in the biotechnology industry. Simple, sensitive, and versatile tools that detect the fibrillar conformation of amyloidogenic proteins are thus of great importance. We have developed a negatively charged conjugated polyelectrolyte that displays different characteristic optical changes, detected visually or by absorption and emission, depending on whether the protein with which it forms a complex is in its native state or amyloid fibril conformation. This simple, rapid, and novel methodology was applied here to two amyloidogenic proteins, insulin and lysozyme, and its validity for detection of their fibrillar conformation was verified by currently used methods Such as circular dichroism, transmission electron microscopy, and Congo red absorption.