IDENTIFICATION OF SOME BACTERIAL-CELL COMPONENTS BY FT-IR SPECTROSCOPY
IDENTIFICATION OF SOME BACTERIAL-CELL COMPONENTS BY FT-IR SPECTROSCOPY
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DOI：
10.1016/0378-1097(94)00529-z
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发表时间：
1995-02-01
影响因子：
2.1
通讯作者：
NAUMANN, D
中科院分区：
文献类型：
--
作者：
HELM, D;NAUMANN, D
傅立叶变换红外光谱（FT-IR）可以检测和鉴定完整细菌的某些特定细胞成分。典型的标记带用于鉴定这些细菌细胞组分。多肽胶囊中检测到几种芽孢杆菌的α-螺旋结构的典型频带和强羧酸伸缩振动。使用吡啶二羧酸的标记带发现梭菌和杆菌中的内生孢子的形成。一些芽孢杆菌菌株的光谱显示聚-β-羟基丁酸颗粒，胶囊和内生孢子同时表达。
Some particular cell components can be detected and identified by Fourier Transform infrared spectroscopy (FT-IR) of intact bacteria. Typical marker bands were used to identify these bacterial cell components. Polypeptide capsules were detected in several Bacillus species by a band typical for alpha-helical structures and by strong carboxylate stretching vibrations. Formation of endospores in clostridia and bacilli was discovered using marker bands for dipicolinic acid. Spectra of some Bacillus strains showed expression of poly-beta-hydroxybutyric acid granules, capsules and endospores simultaneously.