Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing.
Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing.
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DOI：
10.3390/antibiotics4040455
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发表时间：
2015-10-29
期刊：
影响因子：
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通讯作者：
Zare RN
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文献类型：
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作者：
Kim SC;Cestellos-Blanco S;Inoue K;Zare RN
细菌感染的有效治疗依赖于及时的诊断和合理的抗生素药物处方。药敏试验(AST)是最关键的实验程序之一，为选择有效的抗生素及其剂量提供了基线信息。然而，传统方法需要较长的孵化时间或较高的仪器成本才能获得测试结果。我们提出了一种芯片实验室的方法，以一种简单、经济和快速的方式进行AST。我们的检测平台在微流控设备(20×20 mm)上将标准的肉汤微量稀释法微型化，该设备可产生抗生素浓度梯度，并将含有抗生素的培养液输送到8个30-NL小室进行细胞培养。用氨苄西林或链霉素处理过的模式菌株(E.ColiATCC 25922)的20个μL样品进行检测，我们的方法可以在3小时内测定出与微量稀释试验一致的最低抑菌浓度，比标准方法快近10倍。
Effective treatment of bacterial infection relies on timely diagnosis and proper prescription of antibiotic drugs. The antimicrobial susceptibility test (AST) is one of the most crucial experimental procedures, providing the baseline information for choosing effective antibiotic agents and their dosages. Conventional methods, however, require long incubation times or significant instrumentation costs to obtain test results. We propose a lab-on-a-chip approach to perform AST in a simple, economic, and rapid manner. Our assay platform miniaturizes the standard broth microdilution method on a microfluidic device (20 × 20 mm) that generates an antibiotic concentration gradient and delivers antibiotic-containing culture media to eight 30-nL chambers for cell culture. When tested with 20 μL samples of a model bacterial strain (E. coli ATCC 25922) treated with ampicillin or streptomycin, our method allows for the determination of minimum inhibitory concentrations consistent with the microdilution test in three hours, which is almost a factor of ten more rapid than the standard method.