Versatility of biotin-labeled lectins and avidin-biotin-peroxidase complex for localization of carbohydrate in tissue sections.
Versatility of biotin-labeled lectins and avidin-biotin-peroxidase complex for localization of carbohydrate in tissue sections.
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生物素标记的凝集素和抗生物素蛋白-生物素-过氧化物酶复合物的多功能性，用于定位组织切片中的碳水化合物。
DOI：
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发表时间：
1982
影响因子：
3.2
通讯作者：
L. Raine
中科院分区：
文献类型：
--
作者：
S. Hsu;L. Raine
组织中的碳水化合物残基可以通过一种非常敏感、特异和简单的技术来定位，该技术利用生物素标记的凝集素，然后利用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）。花生受体在良、恶性组织中的分布有很大差异。正常结肠粘膜偶见核上胞浆内的小斑点染色，而结肠腺癌的肿瘤细胞则倾向于呈弥漫性胞浆或膜染色。正常乳腺导管上皮在管腔边缘显示膜染色，而乳腺髓样癌显示明显的膜和细胞质染色。使用这种抗生物素蛋白-生物素-过氧化物酶复合物技术可能有助于了解肿瘤发生的过程，以及提供一种敏感的方法来确定恶性转化的早期阶段。
Carbohydrate residues in tissue can be localized by a very sensitive, specific, and simple technique, utilizing a biotin-labeled lectin followed by an avidin-biotin-peroxidase complex (ABC). Distribution of peanut receptors in benign and malignant tissues was found to be quite different. Normal colonic mucosa occasionally shows small dot staining in the supranuclear cytoplasm, while the tumor cells of colonic adenocarcinoma tend to show diffuse cytoplasmic or membranous staining. Normal breast ductal epithelium shows membranous staining at luminal borders, while medullary carcinoma of the breast shows distinct membranous and cytoplasmic staining. The use of this avidin-biotin-peroxidase complex technique may contribute to understanding the process of tumorigenesis as well as providing a sensitive method to determine early stages of malignant transformation.