Sequence and structure of a phenylalanine ammonia-lyase gene from Glycine max.
Sequence and structure of a phenylalanine ammonia-lyase gene from Glycine max.
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DOI：
10.3109/10425179109020788
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
Vodkin, L O
中科院分区：
文献类型：
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作者：
Frank, R L;Vodkin, L O
从大豆中克隆了花青素生物合成关键酶苯丙氨酸解氨酶(PAL)基因。目的是获得一个分子探针来研究该基因家族在大豆中的组织结构，并研究该系统中花青素生物合成途径中存在的新的调控机制。在噬菌体载体lambda Charon 35中构建了大豆基因组文库。利用四季豆PAL基因克隆对文库进行筛选，获得了两个PAL基因。对其中一个基因进行了全序列测定，并与菜豆的PAL2基因进行了序列同源性分析。基因组分析表明，大豆PAL序列是一个只有2-3个成员的小基因家族。具有代表性的PAL基因序列(PAL1)具有2142个碱基对的编码区，分布在两个外显子之间。单个内含子全长1519个碱基，共分131个密码子。
The gene encoding a key enzyme in anthocyanin biosynthesis, phenylalanine ammonia-lyase (PAL), was cloned from soybean (Glycine max). The purpose was to obtain a molecular probe to study the organization of this gene family in soybean and to examine novel regulatory mechanisms present in the anthocyanin biosynthetic pathway of this system. A soybean genomic library was constructed in the bacteriophage vector lambda Charon 35. A PAL cDNA clone from Phaseolus vulgaris was used in screening the library, and two PAL genes were isolated. One gene was sequenced entirely and analyzed by sequence homology to the PAL2 gene of Phaseolus vulgaris. Genomic analysis indicates that PAL sequences of Glycine max exist as a small gene family consisting of only two to three members. The representative PAL gene sequenced (PAL1) has a coding region of 2142 basepairs divided among two exons. The single intron is 1519 basepairs and splits the 131st codon.