A study of multi-ligand beta-lactoglobulin complex formation.
A study of multi-ligand beta-lactoglobulin complex formation.
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DOI：
10.1016/j.foodchem.2014.05.109
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发表时间：
2014-12
期刊：
影响因子：
8.8
通讯作者：
Jie Zhang;Xiaoming Liu;M. Subirade;P. Zhou;L. Liang
中科院分区：
文献类型：
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作者：
Jie Zhang;Xiaoming Liu;M. Subirade;P. Zhou;L. Liang
β-乳球蛋白（β-LG）是主要的乳清蛋白，具有多个结合配体的位点。大多数β-LG-配体相互作用的研究都集中在与单一配体（如α-生育酚、白藜芦醇或叶酸）形成和解离蛋白质复合物。在这项研究中，使用蛋白质内源性荧光猝灭分析了多种生物活性化合物同时与β-LG结合的可能性。发现β-LG可以同时结合两个或三个配体，尽管配体加入的顺序影响结合亲和力。结合荧光光谱和高效液相色谱的结果，这三个配体的物理化学性质的β-LG的影响进行了讨论。本研究中获得的数据表明开发多种活性化合物的基于β-LG的载体的可行性。
Beta-lactoglobulin (β-LG), the principal whey protein, possesses multiple sites for binding ligands. Most studies of β-LG-ligand interactions have focused on the formation and dissociation of protein complexes with single ligands, such as α-tocopherol, resveratrol or folic acid. In this study, the possibility of a plurality of bioactive compounds binding simultaneously to β-LG was analysed using protein intrinsic fluorescence quenching. It was found that β-LG could bind two or three ligands simultaneously, although the sequence in which the ligands were added affected binding affinity. The impact of binding to β-LG on physicochemical properties of these three ligands is discussed in view of fluorescence spectroscopy and high performance liquid chromatography results. The data obtained in this study suggest the feasibility of developing β-LG-based carriers of a plurality of active compounds.