Cariogenic Effects of Probiotic Lactobacillus rhamnosus GG in a Dental Biofilm Model
Cariogenic Effects of Probiotic Lactobacillus rhamnosus GG in a Dental Biofilm Model
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DOI：
10.1159/000355907
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发表时间：
2014-01-01
期刊：
影响因子：
4.2
通讯作者：
Paris, S.
中科院分区：
文献类型：
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作者：
Schwendicke, F.;Doerfer, C.;Paris, S.
益生菌被认为可以抑制变形链球菌(SM)，从而预防龋齿。然而，支持证据很弱，益生菌物种本身可能会导致龋齿。因此，我们将益生菌鼠李糖乳杆菌GG(LGG)与SM进行了比较和组合，并分析了由此导致的牙齿组织中的矿物质损失(Delta Z)。我们模拟了三种生物膜成分(SM、LGG、SM×LGG)、两个病变部位(光滑牙釉质、牙本质洞)和两种营养供应频率(2次/天、6次/天)的连续培养生物膜模型。共有240个牛牙釉质和牙本质样本被切割、抛光和包埋。所有实验程序以独立的副本进行，每个实验每组分配10个样本(最终样本量n=20个/组)。在标本上培养生物膜，连续脉冲供应2%蔗糖培养基和人工唾液。10天后，测定Delta Z和细菌数量。与SM或LGG生物膜相比，SM x LGG生物膜的Delta Z显著增加(p<；0.01，Mann-Whitney检验)，牙本质洞中的Delta Z与光滑釉质病变相比显著增加(p<；0.01)。细菌数量在不同物种的生物膜之间没有显著差异(p>；0.05，ANOVA)。频繁的营养供给显著增加了细菌数量(p<；0.01)。与光滑牙釉质相比，牙本质洞中的生物膜含有明显更多的细菌(p<；0.05)。LGG可导致矿物质流失，特别是在牙本质洞和高龋齿条件下。LGG对SM无抑制作用，但对体外龋病过程有一定的促进作用。(C)2014年S.Karger AG，巴塞尔
Probiotic bacteria have been suggested to inhibit Streptococcus mutans (SM) and thus prevent dental caries. However, supporting evidence is weak and probiotic species might be cariogenic themselves. Thus, we compared and combined the probiotic Lactobacillus rhamnosus GG (LGG) with SM and analysed the resulting mineral loss (Delta Z) in dental tissues. We simulated three biofilm compositions (SM, LGG, SM x LGG), two lesion sites (smooth enamel, dentin cavity) and two nutrition supply frequencies (twice/day, 6 times/day) in a multi-station, continuous-culture biofilm model. A total of 240 bovine enamel and dentin samples were cut, polished and embedded. All experimental procedures were performed in independent duplicates, with 10 samples being allocated to each group for each experiment (final sample size n = 20/group). Biofilms were cultured on the specimens and supplied with 2% sucrose medium and artificial saliva in consecutive pulses. After 10 days, Delta Z and bacterial numbers were assessed. SM x LGG biofilms caused significantly increased Delta Z compared with SM or LGG biofilms (p < 0.01, Mann-Whitney test), and Delta Z was significantly in-creased in dentin cavities compared with smooth enamel lesions (p < 0.01). Bacterial numbers did not significantly differ between biofilms of different species (p > 0.05, ANOVA). Frequent nutrition supply significantly increased bacterial numbers (p < 0.01). Biofilnns in dentin cavities compared to smooth enamel harboured significantly more bacteria (p < 0.05). LGG induced mineral loss especially in dentin cavities and under highly cariogenic conditions. LGG did not have inhibitory effects on SM, but rather contributed to the caries process in vitro. (C) 2014 S. Karger AG, Basel