TRANSCRIPTIONAL REGULATION OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE BY 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
TRANSCRIPTIONAL REGULATION OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE BY 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
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DOI：
10.1006/bbrc.1995.1951
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发表时间：
1995-07-06
影响因子：
3.1
通讯作者：
TOSCANO, WA
中科院分区：
文献类型：
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作者：
MCNULTY, SE;TOSCANO, WA
研究了 2,3,7,8-四氯二苯并-对二恶英 (TCDD) 对培养的人角质形成细胞中转录和稳态 mRNA 水平、蛋白质表达以及甘油醛-3-磷酸脱氢酶 (GAPDH) 酶活性的作用。 GAPDH 转录随着暴露于 TCDD 的时间而增加，8 天后达到最大值 23 倍。 Northern 印迹分析显示暴露 8 天后 mRNA 增加了 4 倍。在相同的暴露条件下，蛋白质水平增加了 4 倍，脱氢酶活性增加了 5 倍。 GAPDH 通常被认为是一种组成型管家基因，通常用作 Northern 印迹分析的上样对照。我们的数据表明，使用 GAPDH 作为 RNA 对照时应谨慎，尤其是在检查细胞增殖和致癌作用的研究中。 (C) 1995 学术出版社
The action of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) on transcription and steady-state mRNA levels, protein expression, and enzymatic activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in cultured human keratinocytes was examined. GAPDH transcription increased as a function of time of exposure to TCDD, reaching a maximum of 23-fold after 8 days. Northern blot analysis showed a 4-fold increase in mRNA after 8 days exposure. Protein levels were increased 4-fold, and dehydrogenase activity by 5-fold, under the same exposure conditions. GAPDH is commonly thought of as a constitutive housekeeping gene and is often used as a loading control for northern blot analysis. Our data suggest that caution should be used when using GAPDH as an RNA control, especially in studies examining cell proliferation and carcinogenesis. (C) 1995 Academic Press, Inc.