Comparing Scaffold-Free and Fibrin-Based Adipose-Derived Stromal Cell Constructs for Adipose Tissue Engineering: An In Vitro and in Vivo Study
Comparing Scaffold-Free and Fibrin-Based Adipose-Derived Stromal Cell Constructs for Adipose Tissue Engineering: An In Vitro and in Vivo Study
复制标题
比较用于脂肪组织工程的无支架和基于纤维蛋白的脂肪源性基质细胞构建体：体外和体内研究
作者：
F. Verseijden;S. J. P. Sluijs;J. V. van Neck;S. Hofer;S. E. Hovius;G. V. van Osch
脂肪组织工程用于软组织修复的成功受到成脂分化不足、宿主反应不利和血管化不足的限制。在这项研究中，我们研究了无支架球体和纤维蛋白为基础的环境如何影响这些参数在人脂肪源性基质细胞（ASC）为基础的脂肪结构。ASC分化为球状体或基于纤维蛋白的结构。7天后，收集条件培养基，收获球状体/基于纤维蛋白的构建体或将其皮下植入无胸腺小鼠中。植入7天后，基于纤维蛋白的构建体中的血管数量显著高于球状体中的血管数量（93 ± 45 vs. 23 ± 11血管/mm 2），并且对基于纤维蛋白的构建体的炎症反应不太严重。在体外进一步研究了这些结果的原因。我们发现，纤维蛋白为基础的结构中的ASCs分泌显着较高水平的血管生成因子VEGF和HGF和较低水平的炎症细胞因子IL-8。此外，纤维蛋白为基础的结构中的ASCs分泌显着更高水平的瘦素，并显示了2.5倍的脂肪形成转录因子PPARG和脂肪细胞特异性标志物FABP 4，周脂蛋白和瘦素的四倍至五倍的上调。这些结果表明，基于纤维蛋白的ASC构建体可能比无支架球体更适合于基于ASC的脂肪组织重建。
Success of adipose tissue engineering for soft tissue repair has been limited by insufficient adipogenic differentiation, an unfavorable host response, and insufficient vascularization. In this study, we examined how scaffold-free spheroid and fibrin-based environments impact these parameters in human adipose-derived stromal cell (ASC)-based adipose constructs. ASCs were differentiated in spheroids or fibrin-based constructs. After 7 days, conditioned medium was collected and spheroids/fibrin-based constructs were either harvested or implanted subcutaneously in athymic mice. Following 7 days of implantation, the number of blood vessels in fibrin-based constructs was significantly higher than in spheroids (93 ± 45 vs. 23 ± 11 vessels/mm2) and the inflammatory response to fibrin-based constructs was less severe. The reasons for these results were investigated further in vitro. We found that ASCs in fibrin-based constructs secreted significantly higher levels of the angiogenic factors VEGF and HGF and lower levels of the inflammatory cytokine IL-8. Furthermore, ASCs in fibrin-based constructs secreted significantly higher levels of leptin and showed a 2.5-fold upregulation of the adipogenic transcription factor PPARG and a fourfold to fivefold upregulation of the adipocyte-specific markers FABP4, perilipin, and leptin. These results indicate that fibrin-based ASC constructs are potentially more suitable for ASC-based adipose tissue reconstruction than scaffold-free spheroids.
由胚胎干细胞产生的无脚手架的人类心脏组织斑块。
影响因子：
--
作者：
Stevens KR;Pabon L;Muskheli V;Murry CE
通讯作者：
Murry CE
DOI：
10.1152/ajpendo.00272.2007
发表时间：
2008-02-01
影响因子：
5.1
作者：
Bell, Lauren N.;Cai, Liying;Considine, Robert V.
通讯作者：
Considine, Robert V.
影响因子：
2.7
作者：
Huang NF;Lam A;Fang Q;Sievers RE;Li S;Lee RJ
通讯作者：
Lee RJ