Determination of the depth of BODIPY probes in model membranes by parallax analysis of fluorescence quenching
Determination of the depth of BODIPY probes in model membranes by parallax analysis of fluorescence quenching
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DOI：
10.1016/s0005-2736(98)00127-8
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发表时间：
1998-10-15
影响因子：
3.4
通讯作者：
London, E
中科院分区：
文献类型：
--
作者：
Kaiser, RD;London, E
一系列亲脂性和脂质连接的BODIPY（4，4-二氟-4-硼-3a，4a-二氮杂-s-引达省）膜探针的位置通过一系列氮氧自由基标记的脂质（其中氮氧自由基的深度是变化的）对BODIPY荧光的猝灭来分析。当连接到PE的极性头基时，BODIPY在深度上保持在极性头基附近。然而，当连接在自由或磷脂连接的脂肪酰基链的末端时，或者当连接到两个烃链时，我们观察到两个探针群体。一个，通常占主导地位，人口的BODIPY集团“环回”的表面，但第二人口仍然深深嵌入在双层。当连接到脂肪酸或脂肪酰基链时，深层人口似乎位于与其连接到酰基链的点相关的深度。在BODIPY连接到游离脂肪酸，深人口的位置响应于电离的羧基。由于与NBD（7-硝基-2，1，3-苯并恶二唑-4-基）和大多数丹磺酰基不同，酰基链连接的BODIPY基团可以以深埋形式存在，因此我们得出结论，BODIPY连接的酰基链优于NBD或丹磺酰基，这是上级的。连接的酰基链作为膜探针。(C)1998 Elsevier Science B. V.保留所有权利。
The location of a series of lipophilic and lipid-attached BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) membrane probes was analyzed by the quenching of BODIPY fluorescence by a series of nitroxide-labeled lipids in which the depth of the nitroxide group is varied. When attached to the polar headgroup of PE the BODIPY remained near the polar headgroup in depth. However, when attached at the end of free or phospholipid-attached fatty acyl chains, or when attached to two hydrocarbon chains, we observed two probe populations. One, usually dominant, population of BODIPY groups 'looped back' towards the surface, but a second population remained deeply embedded within the bilayer. When attached to a fatty acid or fatty acyl chain, the deep population appeared to locate at a depth related to its point of attachment to the acyl chain. In BODIPY linked to free fatty acids, the location of the deep population responded to the ionization of the carboxyl group. Because, unlike NBD (7-nitro-2,1,3-benzoxadiazol-4-yl) and most dansyl groups, acyl chain linked BODIPY groups can exist in a deeply buried form we conclude that BODIPY linked acyl chains are superior to NBD or dansyl. linked acyl chains as membrane probes. (C) 1998 Elsevier Science B.V. All rights reserved.