The genetic relatedness of Porphyromonas gingivalis clinical and laboratory strains assessed by analysis of insertion sequence (IS) element distribution
The genetic relatedness of Porphyromonas gingivalis clinical and laboratory strains assessed by analysis of insertion sequence (IS) element distribution
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DOI：
10.1034/j.1600-0765.2003.00665.x
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发表时间：
2003-08-01
影响因子：
3.5
通讯作者：
Kitten, T
中科院分区：
文献类型：
--
作者：
Califano, JV;Arimoto, T;Kitten, T
目的：牙龈卟啉单胞菌是牙周炎中常见的致病菌。这种微生物含有大量的插入序列（IS）元件。我们试图确定分布的7个IS元件从菌株W83之间的9个牙龈卟啉单胞菌实验室菌株和9个临床分离株，并使用这些发现来确定菌株relationships.Methods：Southern印迹的BamHI消化的基因组DNA序列插入序列元件ISPg 1 -7进行了探测。结果如下：限制性片段长度多态性（RFLP）模式显示，5个实验室菌株，包括菌株W83，几乎是相同的所有7个IS元素。9株临床分离株中有2株与5株实验室分离株相似。其余4种实验室菌株中有2种具有相似或相同的RFLP图谱。其余两种实验室菌株与临床菌株的相似性有限。四个临床分离株具有相同的RFLP模式的所有七个IS元素。其余3株临床分离株的RFLP图谱具有独特性。一些菌株缺乏1至4个IS元件。类似的应变关系，建议无论IS元素examined.Conclusions：IS元素之间的易位和重组是不够普遍的模糊应变关系，虽然这并不排除这种事件发挥重要作用，让牙龈卟啉单胞菌适应新的环境的可能性。鉴于观察到的遗传多样性水平，在基于W83牙龈卟啉单胞菌基因组数据库得出结论时，检查遗传多样性菌株可能尤为重要。
Objectives: Porphyromonas gingivalis is frequently found in periodontitis lesions. This organism contains a large number of insertion sequence (IS) elements. We sought to determine the distribution of seven IS elements from strain W83 among nine P. gingivalis laboratory strains and nine clinical isolates and to use these findings to determine strain relationships.Methods: Southern blots of BamHI digested genomic DNA digests were probed with insertion sequence elements ISPg1-7. Results: The restriction fragment length polymorphism (RFLP) patterns revealed that five of the nine laboratory strains, including strain W83, were nearly identical for all seven IS elements. Two of nine clinical isolates were similar to the five laboratory strains. Two of the four remaining laboratory strains had similar or identical RFLP patterns. The remaining two laboratory strains had limited similarity to clinical strains. Four of the clinical isolates had identical RFLP patterns for all seven IS elements. The three remaining clinical isolates were unique in their RFLP patterns. Several strains lacked from one to four of the IS elements. Similar strain relationships were suggested regardless of the IS element examined.Conclusions: Transposition and recombination between IS elements are not sufficiently pervasive to obscure strain relationships, though this does not preclude the possibility that such events play an important role in allowing P. gingivalis to adapt to new environments. Given the level of genetic diversity observed, it may be especially important to examine genetically diverse strains when drawing conclusions based on the W83 P. gingivalis genomic database.