Viability interactions, in vivo activity and the G6PD polymorphism in Drosophila melanogaster.
Viability interactions, in vivo activity and the G6PD polymorphism in Drosophila melanogaster.
复制标题
果蝇活力相互作用、体内活性和 G6PD 多态性。
DOI：
10.1093/genetics/106.1.95
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Eanes,WF
中科院分区：
文献类型：
--
作者：
Eanes,WF
Several biochemical studies have suggested that inDrosophila melanogasterthe two common allozymes of G6PD differ in theirin vitroactivities and thermal stabilities. Yet, it remains to be shown that these characterizations reflect actualin vivodifferences and are not artifacts of the biochemical approach. In this study it is shown thatin vivoactivity differences must exist between these two variants. This conclusion arises from the observation that the viability of flies bearing a low activity allele of 6PGD is strongly dependent on the genotype at the G6PD (Zw) locus, whereas no measurable difference in viability can be detected betweenZwgenotypes in a normal activity 6PGD background. These viability interactions are in the direction predicted by the reportedin vitroactivities of the allozymes and the proposed deleterious effects of 6-phosphogluconate accumulation.—In addition, a genetic scheme is used that uncouples and quantifies the effects of viability modifiers in the region of theZwlocus, while homogenizing 98% of theXchromosome. The viability of differentZwgenotypes is measured by examining whole chromosome viabilities relative to theFM6balancer chromosome. The advantages of this particular scheme are discussed.
