Bone Marrow Stromal Cell Transdifferentiation into Oligodendrocyte-Like Cells Using Triiodothyronine as a Inducer with Expression of Platelet-Derived Growth Factor α as a Maturity Marker
Bone Marrow Stromal Cell Transdifferentiation into Oligodendrocyte-Like Cells Using Triiodothyronine as a Inducer with Expression of Platelet-Derived Growth Factor α as a Maturity Marker
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DOI：
10.6091/ibj.11162.2013
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发表时间：
2013-04-01
影响因子：
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通讯作者：
Taheri, Taher
中科院分区：
文献类型：
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作者：
Abbaszadeh, Hojjat-Allah;Tiraihi, Taki;Taheri, Taher
背景：本研究对大鼠骨髓基质细胞（BMSC）少突胶质细胞的功能成熟度进行了研究。方法：从雌性Sprague-Dawley大鼠分离BMSC，检测其纤维连接蛋白、CD106、CD90、Oct-4、CD45等标志物的表达。在诱导前阶段，将BMSC暴露于DMSO和1 μ M全反式维甲酸中，然后用heregulin （HRG）、血小板衍生生长因子AA （pdgfr - α）、成纤维细胞生长因子和T3诱导，获得了BMSC OLC的转分化。采用免疫细胞化学方法检测神经祖细胞（NPC）巢蛋白、神经丝68、神经丝160及胶质原纤维酸性蛋白基因表达。免疫细胞化学检测O4、oligo2、O1和MBP标记物的OLC， RT-PCR检测pdgfr - α基因的表达。结果：我们的结果显示，第4代后，纤维连接蛋白、CD106、CD90、CD45和Oct-4均有表达。使用O1、O4和oligo2标记时，OLC分化率约为71%。同样，pdgfr - α在预少突胶质细胞中表达，而MBP在诱导6天后在少突胶质细胞中表达。结论：BMSC可诱导转分化为成熟的OLC。
Background: The present study investigated the functional maturity of oligodendrocyte derived from rat bone marrow stromal cells (BMSC). Methods: The BMSC were isolated from female Sprague-Dawley rats and evaluated for different markers, such as fibronectin, CD106, CD90, Oct-4 and CD45. Transdifferentiation of OLC from BMSC was obtained by exposing the BMSC to DMSO and 1 mu M all-trans-retinoic acid during the pre-induction stage and then induced by heregulin (HRG), platelet-derived growth factor AA (PDGFR-alpha), fibroblast growth factor and T3. The neuroprogenitor cells (NPC) were evaluated for nestin, neurofilament 68, neurofilament 160 and glial fibrillary acidic protein gene expression using immunocytochemistry. The OLC were assessed by immunocytochemistry for O4, oligo2, O1 and MBP marker and gene expression of PDGFR-alpha was examined by RT-PCR. Results: Our results showed that the fibronectin, CD106, CD90, CD45 and Oct-4 were expressed after the fourth passage. Also, the yield of OLC differentiation was about 71% when using the O1, O4 and oligo2 markers. Likewise, the expression of PDGFR-alpha in pre-oligodendrocytes was noticed, while MBP expression was detected in oligodendrocyte after 6 days of the induction. Conclusion: The conclusion of the study showed that BMSC can be induced to transdifferentiate into mature OLC.