Chorioamniotic membrane senescence: a signal for parturition?
Chorioamniotic membrane senescence: a signal for parturition?
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DOI：
10.1016/j.ajog.2015.05.041
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发表时间：
2015-09-01
影响因子：
9.8
通讯作者：
Menon, Ramkumar
中科院分区：
文献类型：
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作者：
Behnia, Faranak;Taylor, Brandie D.;Menon, Ramkumar
目的：衰老是一种重要的生物学现象，涉及生理和病理过程。我们认为绒毛膜羊膜衰老是与人类分娩相关的一种机制。本研究旨在通过检查绒毛膜羊膜的形态和生化证据来探讨衰老与正常足月分娩之间的关联。 研究设计：从正常足月分娩中收集绒毛膜羊膜；第 1 组：足月临产，第 2 组：足月临产，未临产。通过透射电子显微镜测定衰老相关的形态变化，并通过衰老相关（SA）β-半乳糖苷酶染色研究生化变化。对足月产妇和非足月产妇收集的羊水样本进行 14 种 SA 分泌表型 (SASP) 标记物分析。 结果：与非足月产妇相比，在从足月产妇获得的绒毛膜羊膜中观察到细胞衰老（增大的细胞和细胞器）的形态学证据以及更多数量的 SA β-半乳糖苷酶染色的羊膜和绒毛膜细胞。足月产妇羊水中促炎 SASP 标记物（粒细胞巨噬细胞集落刺激因子、白介素-6 和 -8）的浓度显着高于未临产妇女。相比之下，足月分娩的羊水中，防止细胞死亡的 SASP 因子（eotaxin-1、可溶性 Fas 配体、骨保护素和细胞间粘附分子-1）显着降低。结论：足月分娩妇女的绒毛膜羊膜中衰老的形态和生化特征更为常见。绒毛膜羊膜的衰老也与羊水 SASP 标记物相关。
OBJECTIVE: Senescence is an important biological phenomenon involved in both physiologic and pathologic processes. We propose that chorioamniotic membrane senescence is a mechanism associated with human parturition. The present study was conducted to explore the association between senescence and normal term parturition by examining the morphologic and biochemical evidences in chorioamniotic membranes.STUDY DESIGN: Chorioamniotic membranes were collected from normal term deliveries; group 1: term labor and group 2: term, not in labor. Senescence-related morphologic changes were determined by transmission electron microscopy and biochemical changes were studied by senescence-associated (SA) beta-galactosidase staining. Amniotic fluid samples collected from both term labor and term not in labor were analyzed for 14 SA secretory phenotype (SASP) markers.RESULTS: Morphologic evidence of cellular senescence (enlarged cells and organelles) and a higher number of SA beta-galactosidase-stained amnion and chorion cells were observed in chorioamniotic membranes obtained from women in labor at term, when compared to term not in labor. The concentration of proinflammatory SASP markers (granulocyte macrophage colony-stimulating factor, interleukin-6 and -8) was significantly higher in the amniotic fluid of women in labor at term than women not in labor. In contrast, SASP factors that protect against cell death (eotaxin-1, soluble Fas ligand, osteoprotegerin, and intercellular adhesion molecule-1) were significantly lower in the amniotic fluid samples from term labor.CONCLUSION: Morphologic and biochemical features of senescence were more frequent in chorioamniotic membranes from women who experienced term labor. Senescence of chorioamniotic membranes were also associated with amniotic fluid SASP markers.