Enzymes of Oxidative Metabolism in the Human Eosinophil 1
Enzymes of Oxidative Metabolism in the Human Eosinophil 1
复制标题
人类嗜酸性粒细胞氧化代谢酶 1
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
C. McCall
中科院分区：
文献类型：
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作者：
L. Dechatelet;R. Migler;P. Shirley;D. Bass;C. McCall
总结人嗜酸性粒细胞对NADH和NADPH的吡啶核苷酸氧化酶活性降低。这种活性对NADPH更有活性，并且在差速离心后出现在500 g沉淀和27，000 g沉淀中;嗜酸性粒细胞对两种底物的活性大于中性粒细胞，与吞噬作用后在嗜酸性粒细胞中观察到的更大呼吸爆发一致。参与氧代谢物解毒的其他酶在嗜酸性粒细胞中至少与在中性粒细胞中一样活跃，并且在许多情况下似乎更活跃。这些酶包括过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、葡萄糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶。因此，嗜酸性粒细胞的酶含量将使细胞为涉及产生活性氧化剂的作用做好准备。
Summary Human eosinophils exhibit reduced pyridine nucleotide oxidase activity toward both NADH and NADPH. This activity is more active toward NADPH and appears in both the 500g pellet and 27,000g pellet upon differential centrifugation; activity toward both substrates is greater in eosinophils than neutrophils, consistent with the greater respiratory burst observed in eosinophils upon phagocytosis. Other enzymes involved in detoxification of oxygen metabolites are at least as active in eosinophils as in neutrophils, and in many cases appear to be more active. These include catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase. Thus, the enzyme content of the eosinophil would prepare the cell for a role which involves generation of active oxidizing agents.