Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine
Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine
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DOI：
10.4049/jimmunol.168.1.57
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发表时间：
2002-01-01
影响因子：
4.4
通讯作者：
Ishikawa, H
中科院分区：
文献类型：
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作者：
Hamada, H;Hiroi, T;Ishikawa, H
我们发现，在小鼠小肠的反肠系膜壁的整个长度上可以发现 100-200 个充满紧密排列的淋巴细胞的簇。它们由大的 B 细胞区域（包括生发中心）组成，簇上的上皮细胞含有 M 细胞。大部分B细胞表现出B220(+)CD19(+)CD23(+)IgM(低)IgD(高)CD5(-)M-Mac-1(-)表型，IgA(+)B细胞的组成较小但较多。据我们所知，这些簇是首次鉴定出小鼠小肠中分离的淋巴滤泡（ILF）。 ILF 可在出生后 7 天（BALB/c 小鼠）和 25 天（C57BL/6 小鼠）首次检测到，并且在无菌、无胸腺裸鼠、RAG-2(-/-)、TCR-beta (-/-) 和 Ig mu 链突变体 (mum(-/-)) 小鼠中存在细胞质量与 ILF 相当的淋巴簇，尽管 c-kit(+) 在无菌和无胸腺裸鼠中，细胞数量多于B220(+)细胞，并且RAG-2(-/-)、TCR-β(-/-)和mum(-/-)小鼠中大多数淋巴居民是c-kit(+)B220(-)。 ILF 在经胎盘操作的派尔氏集结 (PP) 缺陷小鼠的后代中正常发育，并且 IL-7R α (-/-) PPnull 小鼠中明显萎缩的 ILF 数量减少。在淋巴毒素 α (-/-) 和 aly/aly 小鼠中均检测不到 ILF 和 PP，这些小鼠保留了发育良好的隐斑 (CP) 和不依赖胸腺的上皮内 T 细胞亚群，而 ELF、PP、CP 和不依赖胸腺的上皮内 T 细胞亚群从常见细胞因子受体 γ 链中消失 突变小鼠。这些发现表明，ILF、PP 和 CP 构成了三种不同组织的肠道相关淋巴组织，位于小鼠小肠固有层中。
We have revealed that 100-200 clusters, filled with closely packed lymphocytes, can be found throughout the length of the anti-mesenteric wall of the mouse small intestine. They are composed of a large B cell area, including a germinal center, and epithelia overlying the clusters contain M cells. A large fraction of B cells displays B220(+)CD19(+)CD23(+)IgM(low)IgD(high)CD5(-)M-Mac-1(-) phenotype, and the composition of IgA(+) B cells is smaller but substantial. To our knowledge, these clusters are the first identification of isolated lymphoid follicles (ILF) in mouse small intestine. ILF can be first detected at 7 (BALB/c mice) and 25 (C57BL/6 mice) days after birth, and lymphoid clusters equivalent in terms of cellular mass to ILF are present in germfree, athymic nude, RAG-2(-/-), TCR-beta (-/-), and Ig mu -chain mutant (mum(-/-)) mice, although c-kit(+) cells outnumber B220(+) cells in germfree and athymic nude mice, and most lymphoid residents are c-kit(+)B220(-) in RAG-2(-/-), TCR-beta (-/-), and mum(-/-) mice. ILF develop normally in the progeny of transplacentally manipulated Peyer's patch (PP)-deficient mice, and decreased numbers of conspicuously atrophied ILF are present in IL-7R alpha (-/-) PPnull mice. Neither ILF nor PP are detectable in lymphotoxin alpha (-/-) and aly/aly mice that retain well-developed cryptopatches (CP) and thymus-independent subsets of intraepithelial T cells, whereas ELF, PP, CP, and thymus-independent subsets of intraepithelial T cells disappear from common cytokine receptor gamma -chain mutant mice. These findings indicate that ILF, PP, and CP constitute three distinct organized gut-associated lymphoid tissues that reside in the lamina propria of the mouse small intestine.