Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins.
Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins.
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0 阶段孢子形成基因 spo0F 的推导产物与 Spo0A、OmpR 和 SfrA 蛋白具有同源性。
DOI：
10.1073/pnas.82.21.7260
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发表时间：
1985
影响因子：
11.1
通讯作者：
Hoch,JA
中科院分区：
文献类型：
--
作者：
Trach,KA;Chapman,JW;Piggot,PJ;Hoch,JA
已在枯草芽孢杆菌DNA的克隆片段上确定了阶段0孢子形成位点spo0F的位置。对spo0F基因和周围区域进行测序，结果显示编码Mr为14，286的蛋白质。推导的蛋白质氨基酸序列与spo0A基因产物的氨基端结构域的同源性为56%。Spo0F蛋白的分子量约为Spo0A蛋白的一半，并且其序列与Spo0A蛋白的氨基末端的一半同源。Spo0A蛋白的同一部分显示出与大肠杆菌的OmpR和SfrA调节蛋白的祖先关系。在任一生物体中编码这些蛋白质的任何基因中的突变是高度多效性的，并且导致膜组分的调节的改变，这表明它们在两种生物体中可能具有相关作用，并且spo0A和spo0F突变体的0期孢子形成缺陷是这种调节系统的间接后果。
The location of the stage 0 sporulation locus spo0F has been determined on a cloned fragment of Bacillus subtilis DNA. The spo0F gene and surrounding region was sequenced and was shown to code for a protein of Mr 14,286. The amino acid sequence of this deduced protein was 56% homologous to the amino-terminal domain of the spo0A gene product. The molecular weight of the Spo0F protein was approximately half that of the Spo0A protein, and its sequence was homologous to the amino-terminal half of the Spo0A protein. This same portion of the Spo0A protein showed ancestral relationship to the OmpR and SfrA regulatory proteins of Escherichia coli. Mutations in any of the genes encoding these proteins in either organism are highly pleiotropic and result in alterations in the regulation of membrane components, suggesting that they may have related roles in both organisms and that the stage 0 sporulation defect of spo0A and spo0F mutants is an indirect consequence of this regulatory system.