Role of the Mycoplasma pneumoniae/Interleukin-8/Neutrophil Axis in the Pathogenesis of Pneumonia.
Role of the Mycoplasma pneumoniae/Interleukin-8/Neutrophil Axis in the Pathogenesis of Pneumonia.
复制标题
肺炎支原体/白细胞介素8/中性粒细胞轴在肺炎发病机制中的作用
DOI：
10.1371/journal.pone.0146377
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Yan Y
中科院分区：
文献类型：
--
作者：
Chen Z;Shao X;Dou X;Zhang X;Wang Y;Zhu C;Hao C;Fan M;Ji W;Yan Y
中性粒细胞浸润是肺炎支原体肺炎(MPP)的典型病理特征。本研究旨在探讨中性粒细胞活性、临床表现和肺炎支原体/白介素8(IL-8)/中性粒细胞轴在MPP发病机制中的作用。共有42名MPP患者被前瞻性纳入研究。检测中性粒细胞活性，包括基质金属蛋白酶-9(MMP9)、髓过氧化物酶(MPO)和中性粒细胞弹性蛋白酶(NE)。收集所有患者和对照组的临床资料。体外检测肺炎支原体感染支气管上皮细胞后不同时间点IL-8的产生，并分析IL-8刺激后中性粒细胞的活性。肺炎支原体DNA高水平组支气管肺泡灌洗液中中性粒细胞百分率明显高于低水平组(P<0.05)。MPP患者IL-8、MMP-9、NE较正常对照组显著升高，治疗后显著降低(P<0.05)。MPO和MMP9分别与发热时间(r=0.332，P<0.05)和住院天数(r=0.342，P<0.05)相关。在体外，肺炎支原体以时间依赖的方式诱导支气管上皮细胞产生IL-8。经IL-8刺激后，中性粒细胞产生MPO、MMP9和NE的量显著高于中性粒细胞。综上所述，肺炎支原体/IL-8/中性粒细胞轴可能在MPP的发病机制中起重要作用。
Neutrophil infiltration is the characteristic pathological feature of M. pneumoniae pneumonia (MPP). This study aimed to explore the associations among neutrophil activity, clinical presentation, and role of the M. pneumoniae/interleukin-8 (IL-8)/neutrophil axis in the pathogenesis of MPP. A total of 42 patients with MPP were prospectively enrolled in the study. Neutrophil activity, including matrix metalloproteinase-9 (MMP-9), myeloperoxidase (MPO), and neutrophil elastase (NE), were measured. Clinical information was collected for all patients and control group. In vitro, IL-8 production was measured at different time points after M. pneumoniae infection of bronchial epithelial cells, and neutrophil activity was analyzed after IL-8 stimulation. The percentage of neutrophil in the bronchoalveolar lavage fluid was higher in the group of patients with high levels of M. pneumoniae DNA than in those with low levels of M. pneumoniae DNA (P < 0.05). IL-8, MMP-9, and NE in patients with MPP significantly increased compared with controls and decreased after treatment (P < 0.05). MPO and MMP-9 were associated with duration of fever (r = 0.332, P < 0.05) and length of stay (r = 0.342, P < 0.05), respectively. In vitro, M. pneumoniae induced IL-8 production by bronchial epithelial cells in a time dependent manner. MPO, MMP-9 and NE production by neutrophils significantly increased compared with medium controls after IL-8 stimulation. In summary, the M. pneumoniae/IL-8/neutrophil axis likely plays a vital role in the pathogenesis of MPP.