Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation
Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation
复制标题
DOI：
10.18063/ijb.v6i1.250
复制
发表时间：
2020-01-01
影响因子：
8.4
通讯作者：
Sakai, Shinji
中科院分区：
文献类型：
--
作者：
Gantumur, Enkhtuul;Nakahata, Masaki;Sakai, Shinji
我们报告了一种基于挤出的生物打印方法，其中通过辣根过氧化物酶（HRP）催化的交联消耗由HRP和葡萄糖提供的过氧化氢（H2 O2）来实现挤出生物墨水的稳定化。将含有活细胞、HRP、葡萄糖、具有酚羟基（Ph）基团的藻酸盐和纤维素酶的生物墨水挤出以制造3D水凝胶构建体。晶格和人类鼻子形状的3D结构被成功打印出来，并在细胞培养基中显示出良好的稳定性超过一周。封闭在打印的构建体中的小鼠10 T1/2成纤维细胞在培养7天后保持活力。它还能够将打印的构建体的非细胞粘附表面转换为细胞粘附表面，用于通过具有Ph部分的明胶的后续交联在其上培养细胞。这些结果证明了利用所提出的交联方法进行3D生物打印的可能性。
We report an extrusion-based bioprinting approach, in which stabilization of extruded bioink is achieved through horseradish peroxidase (HRP)-catalyzed cross-linking consuming hydrogen peroxide (H2O2) supplied from HRP and glucose. The bioinks containing living cells, HRP, glucose, alginate possessing phenolic hydroxyl (Ph) groups, and cellulose nanofiber were extruded to fabricate 3D hydrogel constructs. Lattice- and human nose-shaped 3D constructs were successfully printed and showed good stability in cell culture medium for over a week. Mouse 10T1/2 fibroblasts enclosed in the printed constructs remained viable after 7 days of culture. It was also able to switch a non-cell-adhesive surface of the printed construct to cell-adhesive surface for culturing cells on it through a subsequent cross-linking of gelatin possessing Ph moieties. These results demonstrate the possibility of utilizing the presented cross-linking method for 3D bioprinting.