Anti-HIV type 1 cytotoxic T lymphocyte effector activity and disease progression in the first 8 years of HIV type 1 infection of homosexual men.
Anti-HIV type 1 cytotoxic T lymphocyte effector activity and disease progression in the first 8 years of HIV type 1 infection of homosexual men.
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男同性恋者感染 HIV 1 型前 8 年的抗 HIV 1 型细胞毒性 T 淋巴细胞效应活性和疾病进展。
DOI：
10.1089/aid.1995.11.481
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发表时间：
1995
影响因子：
1.5
通讯作者：
Torpey3rd,DJ
中科院分区：
文献类型：
--
作者：
RinaldoJr,CR;Beltz,LA;Huang,XL;Gupta,P;Fan,Z;Torpey3rd,DJ
细胞毒性T淋巴细胞(CTL)可能在宿主抵抗HIV-1感染中发挥重要作用。在这项研究中，我们检测了57名HIV-1感染者对GAG、POL、Env和Tat的特异性循环效应细胞集落刺激因子(CTLE)的反应，其中49人无症状，并记录了自8年血清转换以来的时间。在83%的受试者中，检测到对四种HIV-1基因产物中至少一种的CTLE反应。抗TAT反应的大小和流行率显著低于对GAG、POL和Env的反应。细胞耗竭研究表明，针对HIV-1结构蛋白的裂解活性是由CD8+T细胞介导的，尽管30%的Env特异性裂解是由CD16+自然杀伤细胞介导的。与感染时间较短的受试者相比，感染6年以上的受试者对GAG和POL的抗HIV-1 CTLE反应明显较少。然而，我们发现抗HIV-1 CTLE反应与CD4+或CD8+T细胞计数、CD4+T细胞损失率、HIV-1感染病毒载量、抗病毒药物的使用或随后发展为艾滋病之间没有相关性。我们的结果表明，在感染6年的无症状受试者中，抗HIV-1 CTLE活性相对稳定，并不是疾病进展风险的早期标志。
Cytotoxic T lymphocytes (CTL) may play an important role in host defense against HIV-1 infection. In this study, we examined the responses of circulating effector CTL (CTLe) specific for Gag, Pol, Env, and Tat in 57 HIV-1-infected men, 49 of whom were asymptomatic and had documented time since seroconversion of <8 years. CTLe responses to at least one of the four HIV-1 gene products were detected in 83% of the subjects. The magnitude and prevalence of the anti-Tat responses were significantly less than the responses to Gag, Pol, and Env. Cell depletion studies indicated that the lytic activity against the HIV-1 structural proteins was mediated by CD8+T cells, although 30% of Env-specific lysis was mediated by CD16+natural killer cells. Anti-HIV-1 CTLe responses against Gag and Pol were significantly less in subjects infected for over 6 years as compared to those infected for shorter periods of time. We found no correlation, however, between anti-HIV-1 CTLe responses and either CD4+or CD8+T cell counts, rates of CD4+T cell loss, HIV-1 infectious viral load, use of antiviral medications, or subsequent progression to AIDS. Our results indicate that anti-HIV-1 CTLe activity is relatively stable in asymptomatic subjects infected <6 years, and is not an early marker for risk of disease progression.